Green propolis extract attenuates acute kidney injury and lung injury in a rat model of sepsis

Autor(es)

Duarte Silveira, Marcelo Augusto ^[1] ; Condor Capcha, Jose Manuel ^[1] ; Sanches, Talita Rojas ^[1] ; de Sousa Moreira, Roberto ^{[1, 2]} ; Garnica, Margot S. ^[3] ; Shimizu, Maria Heloisa ^[1] ; Berretta, Andresa ^[4] ; Teles, Flavio ^[5] ; Noronha, Irene L. ^[3] ; Andrade, Lucia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Med, Div Nephrol, Lab Basic Sci Renal Dis, Sao Paulo - Brazil

^[2] Univ Goias, Catalao - Brazil

^[3] Univ Sao Paulo, Sch Med, Lab Cellular Genet & Mol Nephrol, Div Nephrol, Sao Paulo - Brazil

^[4] Apis Flora Ind & Comercial Ltda, Lab Pesquisa Desenvolvimento & Inovacao PD&I, Ribeirao Preto - Brazil

^[5] Univ Fed Alagoas, Sch Med, Maceio, Alagoas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 11, n. 1 MAR 15 2021.
• Citações Web of Science: 1

Resumo

Sepsis is the leading cause of acute kidney injury (AKI) and lung injury worldwide. Despite therapeutic advances, sepsis continues to be associated with high mortality. Because Brazilian green propolis (GP) has promising anti-inflammatory, antioxidant, and immunomodulatory properties, we hypothesized that it would protect kidneys and lungs in rats induced to sepsis by cecal ligation and puncture (CLP). Male Wistar rats were divided into groups-control (sham-operated); CLP (CLP only); and CLP+GP (CLP and treatment with GP at 6 h thereafter)-all receiving volume expansion and antibiotic therapy at 6 h after the procedures. By 24 h after the procedures, treatment with GP improved survival, attenuated sepsis-induced AKI, and restored renal tubular function. Whole-blood levels of reduced glutathione were higher in the CLP+GP group. Sepsis upregulated the Toll-like receptor 4/nuclear factor-kappa B axis in lung and renal tissues, as well as increasing inflammatory cytokine levels and macrophage infiltration; all of those effects were attenuated by GP. Treatment with GP decreased the numbers of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling-positive cells in renal and lung tissue, as well as protecting the morphology of the renal mitochondria. Our data open the prospect for clinical trials of the use of GP in sepsis. (AU)

• Processo FAPESP: 10/19012-0 - Avaliação da terapia com células-tronco hematopoiéticas na doença renal crônica em cães
• Beneficiário: Lucia da Conceição Andrade
• Modalidade de apoio: Auxílio à Pesquisa - Temático