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Exercise-Induced Modulation of Angiotensin II Responses in Femoral Veins From 2-Kidney-1-Clip Hypertensive Rats

Texto completo
Autor(es):
Chies, Agnaldo Bruno [1] ; Spadella, Maria Angelica [2] ; de Oliveira, Priscila Ramos [1] ; Domeniconi, Raquel Fantin [3] ; de Mello Santos, Talita [3] ; Moreira, Roseli Peres [4] ; Rosales, Carla B. [5, 6] ; Casarini, Dulce Elena [4] ; Navar, Luis Gabriel [5, 6]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Marilia Med Sch, Pharmacol Lab, Marilia, SP - Brazil
[2] Marilia Med Sch, Human Embryol Lab, Marilia, SP - Brazil
[3] UNESP, Inst Biociences, Dept Anat, Botucatu, SP - Brazil
[4] Univ Fed Sao Paulo, Escola Paulista Med, Div Nephrol, Dept Med, Sao Paulo - Brazil
[5] Tulane Univ, Sch Med, Dept Physiol, New Orleans, LA 70112 - USA
[6] Tulane Univ, Sch Med, Hypertens & Renal Ctr Excellence, New Orleans, LA 70112 - USA
Número total de Afiliações: 6
Tipo de documento: Artigo Científico
Fonte: FRONTIERS IN PHYSIOLOGY; v. 12, APR 7 2021.
Citações Web of Science: 0
Resumo

The present study investigated the angiotensin II (Ang II) responses in rat femoral veins taken from 2-kidney-1clip (2K1C) hypertensive rats at 4 weeks after clipping, as well as the effects of exercise on these responses. In this manner, femoral veins taken from 2K1C rats kept at rest or exposed to acute exercise or to exercise training were challenged with Ang II or endothelin-1 (ET-1) in organ bath. Simultaneously, the presence of cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) were determined in these preparations by western blotting. In these experiments, femoral veins exhibited subdued Ang II responses. However, after nitric oxide (NO) synthesis blockade, the responses were higher in the femoral veins taken from animals kept at rest {[}0.137(0.049-0.245); n = 10] than those obtained in trained animals kept at rest {[}0.008(0.001-0.041); n = 10] or studied after a single bout of exercise {[}0.001(0.001-0.054); n = 11]. In preparations in which, in addition to NO synthesis, both the local production of prostanoids and the action of ET-1 on type A (ETA) or B (ETB) receptors were inhibited, the differences induced by exercise were no longer observed. In addition, neither ET-1 responses nor the presence of COX-1 and COX-2 in these preparations were modified by the employed exercise protocols. In conclusion, NO maintains Ang II responses reduced in femoral veins of 2K1C animals at rest. However, vasodilator prostanoids as well as other relaxing mechanisms, activated by ETB stimulation, are mobilized by exercise to cooperate with NO in order to maintain controlled Ang II responses in femoral veins. (AU)

Processo FAPESP: 21/02532-6 - Modulação induzida por exercício sobre as respostas de veias femorais de ratos hipertensos 2-rim-1-clip à angiotensina II
Beneficiário:Agnaldo Bruno Chies
Linha de fomento: Auxílio à Pesquisa - Publicações científicas - Artigo
Processo FAPESP: 13/22655-9 - Modulação das respostas de veias femorais à angiotensina II exercida pelo exercício em ratos hipertensos 2R1C
Beneficiário:Agnaldo Bruno Chies
Linha de fomento: Auxílio à Pesquisa - Regular