Strontium Calcium Phosphate Nanotubes as Bioinspired Building Blocks for Bone Regeneration

Autor(es)

Tovani, Camila B. ^[1] ; Oliveira, Tamires M. ^[1] ; Soares, Mariana P. R. ^[2] ; Nassif, Nadine ^[3] ; Fukada, Sandra Y. ^[2] ; Ciancaglini, Pietro ^[1] ; Gloter, Alexandre ^[4] ; Ramos, Ana P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14040901 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Ciencias Biomol, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Sorbonne Univ, CNRS, Coll France, Lab Chim Mat Condensee Paris, F-75005 Paris - France

^[4] Univ Paris Saclay, Lab Phys Solides, F-91405 Orsay - France

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED MATERIALS & INTERFACES; v. 12, n. 39, p. 43422-43434, SEP 30 2020.
• Citações Web of Science: 1

Resumo

Calcium phosphate (CaP)-based ceramics are the most investigated materials for bone repairing and regeneration. However, the clinical performance of commercial ceramics is still far from that of the native tissue, which remains as the gold standard. Thus, reproducing the structural architecture and composition of bone matrix should trigger biomimetic response in synthetic materials. Here, we propose an innovative strategy based on the use of track-etched membranes as physical confinement to produce collagen-free strontium-substituted CaP nanotubes that tend to mimic the building block of bone, i.e., the mineralized collagen fibrils. A combination of high-resolution microscopic and spectroscopic techniques revealed the underlying mechanisms driving the nanotube formation. Under confinement, poorly crystalline apatite platelets assembled into tubes that resembled the mineralized collagen fibrils in terms of diameter and structure of bioapatite. Furthermore, the synergetic effect of Sr2+ and confinement gave rise to the stabilization of amorphous strontium CaP nanotubes. The nanotubes were tested in long-term culture of osteoblasts, supporting their maturation and mineralization without eliciting any cytotoxicity. Sr2+ released from the particles reduced the differentiation and activity of osteoclasts in a Sr2+ concentration-dependent manner. Their bioactivity was evaluated in a serum-like solution, showing that the particles spatially guided the biomimetic remineralization. Further, these effects were achieved at strikingly low concentrations of Sr2+ that is crucial to avoid side effects. Overall, these results open simple and promising pathways to develop a new generation of CaP multifunctional ceramics that are active in tissue regeneration and able to simultaneously induce biomimetic remineralization and control the imbalanced osteoclast activity responsible for bone density loss. (AU)

• Processo FAPESP: 17/08892-9 - Superfícies bioativas obtidas a partir de filmes Langmuir-Blodgett e biominerais
• Beneficiário: Ana Paula Ramos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/24249-0 - Associação de proteínas osteogênicas a biominerais e óxidos metálicos dopados com terras-raras: interação com sistemas modelo de membrana
• Beneficiário: Camila Bussola Tovani
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 16/21236-0 - Sistemas miméticos de vesículas da matriz extracellular (MVs) para o estudo da regulação do processo de biomineralização: proteolipossomos contendo NPP1 e Anexina V
• Beneficiário: Pietro Ciancaglini
• Modalidade de apoio: Auxílio à Pesquisa - Regular