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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Radiotherapy changes the salivary proteome in head and neck cancer patients: evaluation before, during, and after treatment

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Autor(es):
Ventura, Talita Mendes Oliveira [1] ; Ribeiro, Nathalia Regina [1] ; Taira, Even Akemi [1] ; de Lima Leite, Aline [1] ; Dionizio, Aline [1] ; Rubira, Cassia Maria Fischer [2] ; da Silva Santos, Paulo Sergio [2] ; Buzalaf, Marilia Afonso Rabelo [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Biol Sci, Discipline Biochem, Bauru Sch Dent, Al Octavio Pinheiro Brisolla 9-75, BR-1701290 Bauru, SP - Brazil
[2] Univ Sao Paulo, Dept Surg Stomatol Pathol & Radiol, Discipline Radiol & Stomatol, Bauru Sch Dent, Al Octavio Pinheiro Brisolla 9-75, BR-1701290 Bauru, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: CLINICAL ORAL INVESTIGATIONS; MAY 2021.
Citações Web of Science: 0
Resumo

Objectives Salivary glands are affected during radiotherapy in the head and neck region, leading to a reduction in salivary flow and changes its composition. Besides negatively affecting the oral soft tissues, this can also lead to dental impairment. Thus, we evaluated the effect of radiotherapy in the proteomic profile of the saliva in patients with head and neck cancer (HNC). Materials and methods HNC patients had their saliva collected before (BRT), during (2-5 weeks; DRT), and after (3-4 months; ART) radiotherapy. Saliva was also collected from healthy volunteers (control; C). Samples were processed for proteomic analysis. Results In total, 1055 proteins were identified, among which 46 were common to all groups, while 86, 86, 286, and 395 were exclusively found in C, BRT, DRT, and ART, respectively. Remarkably, alpha-enolase was increased 35-fold DRT compared with BRT, while proline-rich proteins were decreased. ART there was a 16-fold increase in scaffold attachment factor-B1 and a 3-fold decrease in alpha-enolase and several cystatins. When compared with C, salivary proteins of BRT patients showed increases cystatin-C, lysozyme C, histatin-1, and proline-rich proteins Conclusion/clinical revelance Both HNC and radiotherapy remarkably change the salivary protein composition. Altogether, our results, for the first time, suggest investigating alpha-enolase levels in saliva DRT in future studies as a possible biomarker and strategy to predict the efficiency of the treatment. Moreover, our data provide important insights for designing dental products that are more effective for these patients and contribute to a better understanding of the progressive changes in salivary proteins induced by radiotherapy. (AU)

Processo FAPESP: 17/05031-2 - Análise proteômica da película adquirida do esmalte e saliva em pacientes com câncer de cabeça e pescoço submetidos à radioterapia
Beneficiário:Talita Mendes Oliveira Ventura
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 18/17860-6 - Análise proteômica da película adquirida do esmalte em pacientes com câncer de cabeça e pescoço submetidos à radioterapia
Beneficiário:Nathalia Regina Ribeiro
Linha de fomento: Bolsas no Brasil - Iniciação Científica