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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Environmental DNA as a non-invasive alternative for surveying aquatic communities in tank bromeliads

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Autor(es):
Lopes, Carla Martins [1, 2] ; Santos, Marcus Thadeu T. [1, 2, 3] ; Baeta, Delio [1, 2] ; Sabbag, Ariadne Fares [1, 2] ; Haddad, Celio Fernando Baptista [1, 2]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista UNESP, Dept Biodiversidade, Ave 24A, 1515, BR-13506900 Rio Claro, SP - Brazil
[2] Univ Estadual Paulista UNESP, Ctr Aquicultura, Ave 24A, 1515, BR-13506900 Rio Claro, SP - Brazil
[3] Univ Fed Minas Gerais UFMG, Dept Zool, Lab Herpetol, Ave Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: MARINE AND FRESHWATER RESEARCH; MAY 2021.
Citações Web of Science: 0
Resumo

Tank bromeliads provide a freshwater microhabitat for a rich aquatic community, including amphibian species. Some of these amphibians are threatened, among other factors, because their host plants are also threatened. DNA traces left behind by amphibians in the water accumulated in bromeliads provide us a clue to track the presence and geographical distribution of these species in the environment, even when they have a low population abundance. We used an environmental DNA metabarcoding approach to survey three bromeligenous or bromelicolous amphibian species distributed in the Brazilian Atlantic forest. The three species have a low population abundance or are not detected in the field by using traditional methods for up to 100 years. No DNA trace of our target species was identified among the eDNA samples analysed. However, we successfully identified the DNA of one tribe, two genera and nine amphibian species in the bromeliads surveyed. Our results support the high sensitivity and the non-invasive characteristic of eDNA metabarcoding for surveying bromeligenous or bromelicolous amphibians. We also highlight that more amphibian species could eventually use bromeliads as shelter than has been previously thought, evidencing the importance for further investigations about interactions and conservation status of amphibians and bromeliads. (AU)

Processo FAPESP: 16/14054-3 - DNA ambiental aplicado a estudos de monitoramento e conservação de anfíbios anuros na Mata Atlântica
Beneficiário:Carla Martins Lopes
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/50741-7 - Diversidade e conservação dos anfíbios brasileiros
Beneficiário:Célio Fernando Baptista Haddad
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático