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onclinical Toxicological Studies of Brazilian Red Propolis and Its Primary Botanical Source Dalbergia ecastaphyllu

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Autor(es):
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Aldana-Mejia, Jennyfer A. [1] ; Ccana-Ccapatinta, V, Gari ; Squarisi, Iara S. [2] ; Nascimento, Samuel [2] ; Tanimoto, Matheus H. [3] ; Ribeiro, Victor P. [3] ; Arruda, Caroline [3] ; Nicolella, Heloiza [2] ; Esperandim, Tabata [2] ; Ribeiro, Arthur B. [2] ; de Freitas, Karoline S. [2] ; da Silva, Lucas H. D. [2] ; Ozelin, Saulo D. [2] ; Oliveira, Lucas T. S. [2] ; Melo, Alex L. A. [2] ; Tavares, Denise C. [2] ; Bastos, Jairo K. [3]
Número total de Autores: 17
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Lab Pharmacognosy, BR-14040930 Ribeirao Preto, SP - Brazil
[2] Univ Franca, Lab Mutagenesis, BR-14404600 Franca, SP - Brazil
[3] Ccana-Ccapatinta, Gari, V, Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Lab Pharmacognosy, BR-14040930 Ribeirao Preto, SP - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: Chemical Research in Toxicology; v. 34, n. 4, p. 1024-1033, APR 19 2021.
Citações Web of Science: 2
Resumo

Propolis is one of the most widely used products in traditional medicine. One of the most prominent types of Brazilian propolis is the red one, whose primary botanical source is Dalbergia ecastaphyllum (L.) Taub. Despite the potential of Brazilian red propolis for developing new products with pharmacological activity, few studies guarantee safety in its use. The objective of this study was the evaluation of the possible toxic effects of Brazilian red propolis and D. ecastaphyllum, as well as the cytotoxicity assessment of the main compounds of red propolis on tumoral cell lines. Hydroalcoholic extracts of the Brazilian red propolis (BRPE) and D. ecastaphyllum stems (DSE) and leaves (DLE) were prepared and chromatographed for isolation of the major compounds. RP-HPLC-DAD was used to quantify the major compounds in the obtained extracts. The XTT assay was used to evaluate the cytotoxic activity of the extracts in the human fibroblast cell line (GM07492A). The results revealed IC50 values of 102.7, 143.4, and 253.1 mu g/mL for BRPE, DSE, and DLE, respectively. The extracts were also evaluated for their genotoxic potential in the micronucleus assay in Chinese hamster lung fibroblasts cells (V79), showing the absence of genotoxicity. The BRPE was investigated for its potential in vivo toxicity in the zebrafish model. Concentrations of 0.8-6.3 mg/L were safe for the animals, with a LC50 of 9.37 mg/L. Of the 11 compounds isolated from BRPE, medicarpin showed a selective cytotoxic effect against the HeLa cell line. These are the initial steps to determine the toxicological potential of Brazilian red propolis. (AU)

Processo FAPESP: 17/26252-7 - Estudos não clínicos de toxicologia da própolis vermelha e de sua fonte botânica Dalbergia ecastophyllum (L.) Taub.
Beneficiário:Jennyfer Andrea Aldana Mejía
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 17/04138-8 - Realização de estudos químicos, analíticos, biológicos, farmacológicos e tecnológicos para preenchimento das lacunas no desenvolvimento do setor de própolis brasileiro
Beneficiário:Jairo Kenupp Bastos
Linha de fomento: Auxílio à Pesquisa - Temático