Padilla-Gonzalez, Guillermo E.
Da Costa, Fernando B.
Número total de Autores: 3
Afiliação do(s) autor(es):
 Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Lab Pharmacognosy, AsterBioChem Res Team, BR-14040903 Ribeirao Preto, SP - Brazil
 Royal Bot Gardens, Jodrell Lab, Kew Rd, London TW9 3AB - England
 Royal Bot Gardens, Millennium Seed Bank, Haywards Heath RH17 6TN, W Sussex - England
Número total de Afiliações: 3
Tipo de documento:
Citações Web of Science:
The Andean mountains are `center stage' to some of the most spectacular examples of plant diversifications, where geographic isolation and past climatic fluctuations have played a major role. However, the influence of Andean geography and climate as drivers of metabolic variation in Andean plants is poorly elucidated. Here, we studied the influence of those factors on the metabolome of the subtribe Espeletiinae (Asteraceae) using liquid chromatography coupled to high-resolution mass spectrometry data of over two hundred samples from multiple locations. Our results demonstrate that metabolic profiles can discriminate Espeletiinae taxa at different geographic scales, revealing inter- and intraspecific metabolic variations: at the country level, segregation between Colombian and Venezuelan taxa was observed; regionally, between paramo massifs; and locally, between paramo complexes. Metabolic differences in Espeletiinae were mainly explained by geographic isolation, although differences in taxonomic genera, temperature, and elevation, were also important. Furthermore, we found that different species inhabiting the same paramo complex showed stronger chemical similarities than the same species at different locations, corroborating that geographic isolation represents the main driver of metabolic change in Espeletiinae. The current study serves as a starting point to fill in the gaps in how Andean geography and climate have shaped the metabolism of its vegetation and reveal the potential of untargeted metabolomics to study the environmental physiology of plants. (AU)