Comparison of the Photodynamic Action of Porphyrin, Chlorin, and Isobacteriochlorin Derivatives toward a Melanotic Cell Line

Autor(es):Mostrar menos -

Castro, Kelly A. D. F. ^{[1, 2]} ; Ramos, Loyanne ^[2] ; Mesquita, Mariana ^[1] ; Biazzotto, Juliana Cristina ^[2] ; Moura, Nuno M. M. ^[1] ; Mendes, Ricardo F. ^[3] ; Paz, Filipe A. Almeida ^[3] ; Tome, Augusto C. ^[1] ; Cavaleiro, Jose A. S. ^[1] ; Simoes, Mario M. Q. ^[1] ; Faustino, Maria A. F. ^[1] ; Jager, Alessandra Vincenzi ^[2] ; Nakagaki, Shirley ^[4] ; Neves, M. Graca P. M. S. ^[1] ; da Silva, Roberto S. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Aveiro, Dept Chem, LAQV REQUIMTE, P-3810193 Aveiro - Portugal

^[2] Univ Sao Paulo, Fac Pharmaceut Sci Ribeirao Preto, Dept Phys & Chem, BR-14040903 Sao Paulo, SP - Brazil

^[3] Univ Aveiro, CICECO Aveiro Inst Mat, Dept Chem, P-3810193 Aveiro - Portugal

^[4] Univ Fed Parana, Dept Chem, Lab Bioinorgan & Catalysis, BR-81531980 Curitiba, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ACS APPLIED BIO MATERIALS; v. 4, n. 6, p. 4925-4935, JUN 21 2021.
• Citações Web of Science: 0

Resumo

Melanoma is the most dangerous form of skin cancer, with an abrupt growth of its incidence over the last years. It is extremely resistant to traditional treatments such as chemotherapy and radiotherapy, but therapies for this cancer are gaining attention. Photodynamic therapy (PDT) is considered an effective modality to treat several types of skin cancers and can offer the possibility to treat one of the most aggressive ones: melanoma. In this work, the effect of PDT on a melanotic cell line (B16F10 cells) was assessed by exposing cultured cells to 5,10,15-tris(pentafluorophenyl)-20-(4-pyridyl)porphyrin (PS1) and to its chlorin (PS2) and isobacteriochlorin (PS3) corresponding derivatives and red LED light (lambda = 660 +/- 20 nm). The PDT effect in the cells' viability was measured using the MTT assay. The cell apoptosis was quantified by flow cytometry, and the subcellular localization of the photosensitizer was determined by fluorescence microscopy. In addition, the ability of PS2 to generate superoxide radicals was qualitatively assessed by tyrosine nitration. The results show that the efficiency of the PDT process is dependent on the structure of the PS and on their ability to produce singlet oxygen. Besides that, the photoactivation efficiency is highly dependent on the cellular sublocalization of the PS and on its cellular uptake and singlet oxygen production. We also found that the resistant cell line B16F10 has distinctive chlorin, isobacteriochlorin, or porphyrin-specific resistance profiles. Furthermore, it is shown that the highly fluorescent chlorin derivative PS2 can also be considered in imaging diagnostics. (AU)

• Processo FAPESP: 19/19448-8 - Efeito da fotobiomodulação como adendo sinergístico na foto-citotoxicidade de compostos rutênio-ftalocianinas como produtores de óxido nítrico e oxigênio singleto: avaliação bioquímica in vitro em linhagens tumorais bi- e tridimensional (3D)
• Beneficiário: Roberto Santana da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular