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From birth to adulthood: An analysis of the Brazilian lancehead (Bothrops moojeni) venom at different life stages

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Autor(es):
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Hatakeyama, Daniela Miki [1, 2] ; Tasima, Lidia Jorge [1, 2] ; Galizio, Nathalia da Costa [1, 2] ; Serino-Silva, Caroline [1, 2] ; Fabri Bittencourt Rodrigues, Caroline [1, 2] ; Stuginski, Daniel Rodrigues [1] ; Sant'Anna, Savio Stefanini [1] ; Grego, Kathleen Fernandes [1] ; Tashima, Alexandre Keiji [3] ; Nishiduka, Erika Sayuri [3] ; de Morais-Zani, Karen [1, 2] ; Tanaka-Azevedo, Anita Mitico [1, 2]
Número total de Autores: 12
Afiliação do(s) autor(es):
[1] Inst Butantan, Lab Herpetol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Pesquisas Tecnol, Inst Butantan, Interunidades Biotecnol, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Dept Bioquim, Sao Paulo - Brazil
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: PLoS One; v. 16, n. 6 JUN 10 2021.
Citações Web of Science: 0
Resumo

The Brazilian lancehead (Bothrops moojeni) has a wide distribution in Brazil and represents a serious public health hazard. Previous works reported that the symptoms of snakebites caused by B. moojeni juveniles' bites were mainly related to coagulation, while those caused by adults' bites had a more prominent local damage. In this work, we analyzed the venoms of B. moojeni at different life stages to better understand the ontogeny shift in this species. Snakes were grouped by age and sex, and venom pools were formed accordingly. Compositional analyses by one-dimensional electrophoresis (1-DE), chromatography, and mass spectrometry revealed that ontogenetic changes might be mostly related to phospholipase A(2) (PLA(2)) and metalloproteases. Regarding the venoms functional aspect, proteolytic, L-amino acid oxidase, PLA(2), and coagulant in vitro activities were assayed, but only the first and the last ones showed age-related changes, with the venom of snakes up to 1 year-old displaying lower proteolytic and higher coagulant activities, while those from 2 years-old onward presented the opposite relation. The venoms of 3 years-old snakes were exceptions to the compositional and functional pattern of adults as both venoms presented profiles similar to neonates. Sex-related differences were observed in specific groups and were not age-related. In vivo experiments (median lethal dose and hemorrhagic activity) were statistically similar between neonates and adults, however we verified that the adult venom killed mice faster comparing to the neonates. All venoms were mostly recognized by the antibothropic serum and displayed similar profiles to 1-DE in western blotting. In conclusion, the Brazilian lancehead venom showed ontogenetic shift in its composition and activities. Furthermore, this change occurred in snakes from 1 to 2 years-old, and interestingly the venom pools from 3 years-old snakes had particular characteristics, which highlights the importance of comprehensive studies to better understand venom variability. (AU)

Processo FAPESP: 17/16908-2 - Caracterização proteômica e das atividades enzimáticas e fisiopatológicas do veneno das espécies de serpentes que compõem o grupo Bothrops neuwiedi
Beneficiário:Karen de Morais Zani
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/20106-9 - Peptidômica dos venenos de serpentes e aranhas brasileiras
Beneficiário:Alexandre Keiji Tashima
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/25786-0 - Estudo dos inibidores de fosfolipase A2 (PLIs) presentes nos plasmas das serpentes peçonhentas e não-peçonhentas e avaliação da atividade neutralizante destes sobre as atividades da fosfolipase A2 (PLA2) dos venenos de serpentes
Beneficiário:Anita Mitico Tanaka-Azevedo
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/25899-0 - Análise da variabilidade ontogenética do veneno da serpente Bothrops pauloensis
Beneficiário:Lidia Jorge Tasima
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 18/14724-4 - Caracterização proteômica e funcional do veneno de duas espécies de serpentes do gênero Bothrops submetidas à mudança de dieta
Beneficiário:Nathália da Costa Galizio
Linha de fomento: Bolsas no Brasil - Mestrado