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Photodynamic therapy with a new bacteriochlorin derivative: Characterization and in vitro studies

Texto completo
Autor(es):
Pratavieira, Sebastiao [1] ; Uliana, Marciana Pierina [2, 3] ; dos Santos Lopes, Nahryda Samara [4, 5] ; Donatoni, Maria Carolina [3] ; Linares, Diana Rodriguez [2] ; Anibal, Fernanda de Freitas [4, 5] ; de Oliveira, Kleber Thiago [3] ; Kurachi, Cristina [1] ; Oliveira de Souza, Clovis Wesley [4, 5]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sao Carlos Inst Phys, POB 369, BR-13560970 Sao Carlos, SP - Brazil
[2] Univ Fed Integracao Latinoamer, Caixa Postal 2044, BR-85866000 Foz Do Iguacu, PR - Brazil
[3] Univ Fed Sao Carlos, Dept Quim, Rodovia Washington Luis, Km 235-SP 310, BR-13565905 Sao Carlos, SP - Brazil
[4] Univ Fed Sao Carlos, Pos Grad Biotecnol, Rodovia Washington Luis, Km 235-SP 310, BR-13565905 Sao Carlos, SP - Brazil
[5] Univ Fed Sao Carlos, Dept Morfol & Patol, Rodovia Washington Luis, Km 235-SP 310, BR-13565905 Sao Carlos, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Photodiagnosis and Photodynamic Therapy; v. 34, JUN 2021.
Citações Web of Science: 0
Resumo

Photodynamic therapy presents a therapeutic choice that can be utilized to treat diverse neoplasms. In this technique, the critical element is a photosensitive molecule that absorbs light energy and transfers it to molecular oxygen or biological molecules to form reactive oxygen species, thus inducing irreversible damage to target cells and ultimately leading to cell death. Bacteriochlorin derivatives are employed as photosensitizers (PSs), possessing light-absorbing capacity in the near-infrared region. The objective of this study was to prepare a semisynthetic bacteriochlorin from Rhodopseudomonas faecalis and adding Trizma (R) to improve solubility. Cell viability tests, flow cytometry (apoptotic and necrotic cells were identified by Annexin V and propidium iodide), and confocal microscopy were used to evaluate the photoactivity of bacteriochlorin-Trizma (Bchl-T) in fibroblast (HFF-1-control cells) and breast cancer (MCF-7 cells-target cells) cells. At concentrations above 0.5 mu M, Bchl-T demonstrated 80 % cell death, presenting the highest PS interaction (via fluorescence microscopy) with lysosomes, mitochondria, and the endoplasmic reticulum; the cell death type was revealed as apoptosis (via cytometry). Our findings indicated the suitability of Bchl-T for future application in photodynamic therapy against cancer cells by inducing apoptosis. (AU)

Processo FAPESP: 09/54035-4 - EMU: facility para estudos avançados de materiais nanoestruturados e biossistemas / FAMA
Beneficiário:Igor Polikarpov
Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
Processo FAPESP: 14/50857-8 - INCT 2014 - de Óptica Básica e Aplicada às Ciências da Vida
Beneficiário:Vanderlei Salvador Bagnato
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 08/57858-9 - Instituto Nacional de Óptica e Fotônica
Beneficiário:Vanderlei Salvador Bagnato
Modalidade de apoio: Auxílio à Pesquisa - Temático
Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
Beneficiário:Vanderlei Salvador Bagnato
Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs