pregulation of TCF21 inhibits migration of adrenocortical carcinoma cell

Autor(es)

Kremer, Jean Lucas ^[1] ; Auricino, Thais Barabba ^[1] ; dos Santos Passaia, Barbara ^[1] ; Lotfi, Claudimara Ferini Pacicco ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: HORMONES & CANCER; v. 12, n. 1 JUL 23 2021.
• Citações Web of Science: 0

Resumo

Background Adrenocortical carcinomas (ACC) are rare and aggressive cancer. Our previous study has revealed that the transcription factor 21, TCF21, is downregulated in ACC and regulates steroidogenic factor 1 (SF-1) binding to the SF-1 E-box promoter. In addition, it could be found that TCF21 is a predictor of overall survival (OS) in adult carcinomas. Methods In this study, it was investigated the correlation between TCF21 expression and the promoter methylation status in adrenocortical tumor cells, carcinomas and adenoma. The biological function and potential molecular mechanism of TCF21 restoration in migration and invasion of ACC cells was examined. Results We could be demonstrated a negative correlation between the level of TCF21 expression and methylation of its promoter in adenoma and carcinoma cells indicating the epigenetic control of TCF21 expression. It was also demonstrated that the expression of TCF21 inhibits migration and invasion in the ACC cell line, H295R cells, using plasmid transfection to express TCF21. Furthermore, it could be investigated the TCF21 function as tumor suppressor probably through Kisspeptin 1 (KISS-1) expression and epithelial-mesenchymal transition (EMT) reversion, as well as the modulation of several metalloproteinases in ACC cells. Conclusions Our results suggest that enhancement of TCF21 expression levels may be a potential strategy to revert invasive abilities in adrenocortical carcinomas. (AU)

• Processo FAPESP: 16/17285-6 - Análise da regulação epigenética do fator de transcrição POD1/TCF21 em culturas de células de tumores adrenocorticais, e seu papel na migração e invasão celular.
• Beneficiário: Jean Lucas Kremer
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/19035-2 - Estudo morfológico, funcional e molecular da diferenciação celular do córtex adrenal: o papel do gene SOCS3
• Beneficiário: Claudimara Ferini Pacicco Lotfi
• Modalidade de apoio: Auxílio à Pesquisa - Regular