MagC is a NplC/P60-like member of the alpha-2-macroglobulin Mag complex of Pseudomonas aeruginosa that interacts with peptidoglycan

Autor(es)

Zouhir, Samira ^[1] ; Contreras-Martel, Carlos ^[2] ; Maragno Trindade, Daniel ^[1] ; Attree, Ina ^[3] ; Dessen, Andrea ^{[1, 2]} ; Macheboeuf, Pauline ^[2]

Afiliação do(s) autor(es)

^[1] CNPEM, Brazilian Biosci Natl Lab LNBio, Campinas - Brazil

^[2] Univ Grenoble Alpes, CNRS, CEA, IBS, Grenoble - France

^[3] Univ Grenoble Alpes, CNRS, INSERM, Unite Biol Cellulaire & Infect, CEA, Grenoble - France

• Tipo de documento: Artigo Científico
• Fonte: FEBS Letters; v. 595, n. 15, p. 2034-2046, AUG 2021.
• Citações Web of Science: 0

Resumo

Bacterial alpha-2 macroglobulins (A2Ms) structurally resemble the large spectrum protease inhibitors of the eukaryotic immune system. In Pseudomonas aeruginosa, MagD acts as an A2M and is expressed within a six-gene operon encoding the MagA-F proteins. In this work, we employ isothermal calorimetry (ITC), analytical ultracentrifugation (AUC), and X-ray crystallography to investigate the function of MagC and show that MagC associates with the macroglobulin complex and with the peptidoglycan (PG). However, the catalytic residues of MagC display an inactive conformation that could suggest that it binds to PG but does not degrade it. We hypothesize that MagC could serve as an anchor between the MagD macroglobulin and the PG and could provide stabilization and/or regulation for the entire complex. (AU)

• Processo FAPESP: 13/01962-0 - Estrutura e função de fatores de virulência bacterianos
• Beneficiário: Samira Zouhir
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/12436-9 - ANTIBIO-BAC: a parede bacteriana como alvo para o desenvolvimento de novos agentes antimicrobianos
• Beneficiário: Andrea Dessen de Souza e Silva
• Modalidade de apoio: Auxílio à Pesquisa - Programa SPEC
• Processo FAPESP: 11/52067-6 - Estruturação de complexos macromoleculares da parede bacteriana: biossíntese e virulência
• Beneficiário: Andrea Dessen de Souza e Silva
• Modalidade de apoio: Auxílio à Pesquisa - Programa SPEC