Photobiomodulation therapy does not depend on the differentiation of dental pulp cells to enhance functional activity associated with angiogenesis and mineralization

Autor(es)

Chimello-Sousa, Daniela Thomazatti ^[1] ; Lavez, Geovane Praxedes ^[1] ; Fernandes, Roger Rodrigo ^[2] ; Tavares, Milla Sprone ^[2] ; Rosa, Adalberto Luiz ^[2] ; Siessere, Selma ^[1] ; Hallak Regalo, Simone Cecilio ^[1] ; Bombonato-Prado, Karina Fittipaldi ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Basic & Oral Biol, Bone Res Lab, Ave Cafe S-N, BR-14040904 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral & Maxillofacial Surg & Periodontol, Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Lasers in Medical Science; v. 36, n. 9 AUG 2021.
• Citações Web of Science: 0

Resumo

The purpose of this study is to analyze the influence of InGaAlP diode laser (660 nm) with or without an odontogenic medium (OM) in the functional activity of OD-21 cells. Undifferentiated OD-21 pulp cells were cultivated with or without OM and divided into four groups (n = 5): nonirradiated control (C -), nonirradiated + OM (C +), irradiated (L -), and irradiated + OM (L +). Laser application was performed in two sessions of a 24-h interval with an irradiance of 11.3 mW/cm2, energy density of 1 J/cm(2), and total cumulative energy/well of 4.6 J. Cell proliferation, VEGF-164 expression, mineralization, and expression of Alp, Runx2, and Dmp1 genes, as well as immunolocalization of RUNX2 and MEPE proteins, were evaluated. Data were analyzed by statistical tests (alpha = 0.05). All studied groups showed a similar increase in cell proliferation with or without OM. After 7 and 10 days, a significatively higher concentration of VEGF-164 in L - group when compared to C - group was observed. A significant increase in mineralized nodules in the L + was noted when compared to C + in the same conditions. Photobiomodulation upregulated significantly Runx2 and Dmp1 expression after 10 days in L - and after 7 days in L + , with downregulation of Dmp1 after 10 days in L + group. Immunolocalization of RUNX2 and MEPE was expressive after 7 days of culture in the cytoplasm adjacent to the nucleus with a decrease after 10 days, regardless of the presence of OM. Photobiomodulation enhances metabolism associated with angiogenesis, gene expression, and mineralization regardless of the odontogenic medium in OD-21 cells. (AU)

• Processo FAPESP: 10/50086-0 - Avaliação in vitro de células-tronco da polpa dental (OD-21) após irradiação com laser de arsenieto de gálio-alumínio (AsGaAl)
• Beneficiário: Simone Cecilio Hallak Regalo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/58600-5 - Avaliacao "in vitro" de celulas-tronco da polpa dental (od-21) apos irradiacao com laser de arsenieto de galio-aluminio (asgaal).
• Beneficiário: Daniela Thomazatti Chimello de Sousa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado