Protoporphyrin IX (PpIX) loaded PLGA nanoparticles for topical Photodynamic Therapy of melanoma cells

Autor(es)

da Silva, Darlan Barbosa ^[1] ; da Silva, Carolina Loureiro ^[1] ; Davanzo, Nathalia Nossi ^[2] ; Souza, Rodrigo da Silva ^[3] ; Correa, Rodrigo Jose ^[3] ; Tedesco, Antonio Claudio ^[2] ; Riemma Pierre, Maria Bernadete ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Rio de Janeiro, Sch Pharm, Av Carlos Chagas Filho 373, BR-21941902 Rio De Janeiro, RJ - Brazil

^[2] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem, Photobiol & Photomed Res Grp, Av Bandeirantes 3900, BR-14040901 Vila Monte Alegre - Brazil

^[3] Univ Fed Rio de Janeiro, Inst Quim, Rio De Janeiro - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 35, SEP 2021.
• Citações Web of Science: 0

Resumo

Introduction: Nanoparticles (Np) can increase drug efficacy and overcome problems associated with solubility and aggregation in a solution of PpIX. Purpose: Evaluate if Np interferes in the photophysical and photobiological capacity of the PpIX comparing with free PpIX intended for topical PDT of melanoma. Methods: In vitro photophysical evaluation of Np-PpIX was carried out through singlet oxygen (O-1(2)) quantum yield. In vitro cytotoxicity and phototoxicity assays have used murine melanoma cell culture. Results: The quantum yield of singlet oxygen has shown that Np did not influence the formation capacity of this reactive species. In the dark, all PpIX-Nps concentrations were less cytotoxic compared to free drugs. At a higher light dose (1500 mJ.cm(2)) 3.91 mu g/mL PpIX had similar % viable cells for free and Np (similar to 34 %) meaning Nps did not interfere in the photodynamic effect of PpIX. However, at 7.91 mu g/mL the phototoxicity increased for both (5.8 % viable cells for free versus 21.7 % for Nps). Despite the higher phototoxicity of free PpIX at this concentration, greater cytotoxicity in the dark was obtained (similar to 49 % viable cells for free versus similar to 90 .6 % Np) which means Nps protect the tumor tissue from the photodynamic action of PpIX. Conclusions: Np is a potential delivery system for melanoma skin cancer, since it maintained the photophysical properties of PpIX and excellent in vitro phototoxicity effect against melanoma cells, reducing cell viability similar to 80 % (7.91 mu g/mL PpIX in Nps) and provides safe PDT (due to lower cytotoxicity in the dark). (AU)

• Processo FAPESP: 13/50181-1 - Utilização de nanocarreadores contendo fármacos fotossensibilizantes e outros ativos aplicados à terapia celular e tratamento de patologias do sistema nervoso central
• Beneficiário: Antonio Claudio Tedesco
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/16660-5 - Estudo de nanoemulsões associadas a terapia fotodinâmica no tratamento do câncer de mama
• Beneficiário: Marilia de Freitas Calmon
• Modalidade de apoio: Auxílio à Pesquisa - Regular