Crystal Structure of a Sucrose-6-phosphate Hydrolase from Lactobacillus gasseri with Potential Applications in Fructan Production and the Food Industry

Autor(es)

de Lima, Mariana Z. T. ^[1] ; de Almeida, Leonardo R. ^[1] ; Mera, Alain M. ^[1] ; Bernardes, Amanda ^[1] ; Garcia, Wanius ^[2] ; Muniz, Joao R. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Inst Phys IFSC, BR-13563120 Sao Carlos, SP - Brazil

^[2] Univ Fed ABC UFABC, Ctr Ciencias Nat & Humanas CCNH, BR-09210580 Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Agricultural and Food Chemistry; v. 69, n. 35, p. 10223-10234, SEP 8 2021.
• Citações Web of Science: 0

Resumo

Fructooligosaccharides (FOSs) are polymers of fructose with a prebiotic activity because of their production and fermentation by bacteria that inhabit the gastrointestinal tract and are widely used in the industry and new functional foods. Lactobacillus gasseri stands out as an important homofermentative microorganism related to FOS production, and its potential applications in the industry are undeniable. In this study, we report the production and characterization of a sucrose-6-phosphate hydrolase from L. gasseri belonging to the GH32 family. Apo-LgAs32 and LgAs32 complexed with beta-D-fructose structures were determined at a resolution of 1.94 and 1.84 angstrom, respectively. The production of FOS, fructans, 1-kestose, and nystose by the recombinant LgAs32, using sucrose as a substrate, shown in this study is very promising. When compared to its homologous enzyme from Lactobacillus reuteri, the production of 1-kestose by LgAs32 is increased; thus, LgAs32 can be considered as an alternative in fructan production and other industrial applications. (AU)

• Processo FAPESP: 16/09152-6 - Estudo estrutural e funcional de hidrolases de glicosídeos celulossomais termofílicas envolvidas no processo de hidrólise de biomassa lignocelulósica
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• Processo FAPESP: 17/16291-5 - Caracterização biofísica-estrutural de enzimas termofílicas prospectadas do fungo Thielavia terrestris para degradação de biomassa e geração de produtos biotecnológicos
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