| Texto completo | |
| Autor(es): |
Bagne, Leonardo
[1]
;
Oliveira, Maraiara A.
[1]
;
Pereira, Amanda T.
[1]
;
Caetano, Guilherme F.
[1]
;
Oliveira, Camila A.
[1]
;
Aro, Andrea A.
[1]
;
Chiarotto, Gabriela B.
[1]
;
Santos, Glaucia M. T.
[1]
;
Mendonca, Fernanda A. S.
[1]
;
Santamaria-Jr, Milton
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] Univ Ctr Herminio Ometto Fdn FHO, Grad Program Biomed Sci, Araras, SP - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 109, n. 12, p. 2104-2116, DEC 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
This study aimed to investigate the application of low-intensity electrostimulation (ES) and electromagnetic stimulation (EM) associated with bioactive glass (BG) or allogeneic grafts (BB) in bone regeneration. A cell viability test on osteoblasts (UMR-106) was performed in the presence of BB and BG grafts associated with ES (10 mu A/5 min) and EM (500 Hz/2 min). Critical defects (25 mm(2)) in calvaria were generated in male Wistar rats, and bone regeneration was evaluated on the 30th, 60th, and 120th days after surgery. Cell proliferation increased with the application of ES in both grafts and after EM with BG. Bone remodeling was more effective using the allogeneic graft in both therapies, with increased angiogenesis, osteoblast proliferation, and OPN expression in the BB + EM group. A higher number of osteoblasts and osteoclasts, and an increase in bone sialoprotein, Runx-2, and Opn gene expression were found in the BB + ES group. The BG graft associated with EM therapy had an increased proliferation of osteoblasts and increased expression of Runx-2 and Opn. Groups that had BG and ES therapy had increased numbers of osteoblasts, osteoclasts, and increased OPN expression. The expression of voltage-gated calcium channels increased in groups with ES, while calmodulin expression increased in therapies without grafting. ES and EM therapies favored the repair of bone defects upon grafting by improving angiogenesis, osteogenic gene expression, and tissue reorganization. Despite activating different pathways, both therapies increased the intracellular concentrations of calmodulin, leading to cell proliferation and bone regeneration. (AU) | |
| Processo FAPESP: | 16/23237-4 - Caracterização do perfil osteogênico em defeitos ósseos com enxertia submetidos a terapia com corrente elétrica de baixa intensidade e eletroestimulação magnética |
| Beneficiário: | Milton Santamaria Júnior |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/13361-2 - Caracterização do perfil osteogênico em defeitos ósseos com enxertia submetidos a terapia com corrente elétrica de baixa intensidade e eletroestimulação magnética |
| Beneficiário: | Leonardo Bagne |
| Modalidade de apoio: | Bolsas no Brasil - Programa Capacitação - Treinamento Técnico |