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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Plasma membrane permeabilization to explain erythrosine B phototoxicity on in vitro breast cancer cell models

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Autor(es):
Bistaffa, Maria J. [1] ; Camacho, Sabrina A. [2, 1] ; Melo, Carlos F. O. R. [1] ; Catharino, Rodrigo R. [3] ; Toledo, Karina A. [1, 4] ; Aoki, Pedro H. B. [1]
Número total de Autores: 6
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, UNESP, Sch Sci Humanities & Languages, BR-19806900 Assis, SP - Brazil
[2] Univ Sao Paulo, Sao Carlos Inst Phys, IFSC, BR-13566590 Sao Carlos, SP - Brazil
[3] Univ Estadual Campinas, Sch Pharmaceut Sci, INNOVARE Biomarkers Lab, BR-13083970 Campinas, SP - Brazil
[4] Sao Paulo State Univ, UNESP, Inst Biosci Letters & Exact Sci, BR-15054000 Sao Jose Do Rio Preto, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 223, OCT 2021.
Citações Web of Science: 0
Resumo

Lipid oxidation is ubiquitous in cell life under oxygen and essential for photodynamic therapy (PDT) of carcinomas. However, the mechanisms underlying lipid oxidation in rather complex systems such as plasma membranes remain elusive. Herein, Langmuir monolayers were assembled with the lipid extract of glandular breast cancer (MCF7) cells and used to probe the molecular interactions allowing adsorption of the photosensitizer (PS) erythrosine B and subsequent photooxidation outcomes. Surface pressure (pi) versus area (cm2/mL) isotherms of MCF7 lipid extract shifted to larger areas upon erythrosine incorporation, driven by secondary interactions that affected the orientation of the carbonyl groups and lipid chain organization. Light-irradiation increased the surface area of the MCF7 lipid extract monolayer containing erythrosine owing to the lipid hydroperoxidation, which may further undergo decomposition, resulting in the chain cleavage of phospholipids and membrane permeabilization. Incorporation of erythrosine by MCF7 cells induced slight toxic effects on in vitro assays, differently of the severe phototoxicity caused by light-irradiation, which significantly decreased cell viability by more than 75% at 2.5 x 10-6 mol/L of erythrosine incubated for 3 and 24 h, reaching nearly 90% at 48 h of incubation. The origin of the phototoxic effects is in the rupture of the plasma membrane shown by the frontal (FSC) and side (SSC) light scattering of flow cytometry. Consistent with hydroperoxide decomposition, membrane permeabilization was also confirmed by cleaved lipids detected in mass spectrometry and subsidizes the necrotic pathway of cell death. (AU)

Processo FAPESP: 18/14692-5 - Terapia fototérmica mediada por nanopartículas de ouro: de sistemas modelos de biomembranas ao cultivo in vitro de células tumorais
Beneficiário:Sabrina Aléssio Camacho
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
Beneficiário:Osvaldo Novais de Oliveira Junior
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 16/20576-2 - Fotossensibilização de células derivadas do carcinoma de orofaringe (HEp-2) induzida pela ação do xantênico eritrosina
Beneficiário:Maria Julia Bistaffa
Linha de fomento: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 18/16713-0 - Terapia fotodinâmica: de efeitos moleculares em sistemas modelos a eficiência fotodinâmica em cultivo celular
Beneficiário:Pedro Henrique Benites Aoki
Linha de fomento: Auxílio à Pesquisa - Regular