Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence

Autor(es):Mostrar menos -

Isaac Jordão de Souza ARAÚJO ^[1] ; Mariana Gallante RICARDO ^[2] ; Orisson Ponce GOMES ^[3] ; Priscila Alves GIOVANI ^[4] ; Júlia PUPPIN-RONTANI ^[5] ; Vanessa Arias PECORARI ^[6] ; Elizabeth Ferreira MARTINEZ ^[7] ; Marcelo Henrique NAPIMOGA ^[8] ; Francisco Humberto NOCITI JUNIOR ^[9] ; Regina Maria PUPPIN-RONTANI ^[10] ; Paulo Noronha LISBOA-FILHO ^[11] ; Kamila Rosamilia KANTOVITZ ^[12]

Afiliação do(s) autor(es):Mostrar menos -

^[1] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Restorative Dentistry - Brasil

^[2] Faculdade São Leopoldo Mandic. Dental Research Center - Brasil

^[3] Universidade Estadual Paulista Júlio Mesquita Filho. School of Sciences. Department of Physics - Brasil

^[4] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Pediatric Dentistry - Brasil

^[5] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Restorative Dentistry - Brasil

^[6] Universidade Paulista. School of Dentistry. Department of Bioestatistics - Brasil

^[7] Faculdade São Leopoldo Mandic. Dental Research Center - Brasil

^[8] Faculdade São Leopoldo Mandic. Dental Research Center - Brasil

^[9] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Prosthodontics and Periodontology - Brasil

^[10] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Pediatric Dentistry - Brasil

^[11] Universidade Estadual Paulista Júlio Mesquita Filho. School of Sciences. Department of Physics - Brasil

^[12] Faculdade São Leopoldo Mandic. Dental Research Center - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Oral Research; v. 35, 2021-06-14.

Resumo

Abstract This in vitro study evaluated the impact of TiO2 nanotubes (n-TiO2) incorporated into glass ionomer cement (GIC) on Streptococcus mutans (S. mutans) characteristics at cellular and molecular levels. n-TiO2, synthesized by the alkaline method (20 nm in size), was added to Ketac Molar EasyMix® at 0%, 3%, 5%, and 7% by weight. S. mutans strains were cultured on GIC disks with addition or not of n-TiO2 for 1, 3, and 7 days and the following parameters were assessed: inhibition halo (mm) (n=3/group); cell viability (live/dead) (n=5/group); cell morphology (SEM) (n=3/group); and gene expression by real-time PCR (vicR, covR, gtfB, gtfC, and gtfD) (n=6/group). The data were analyzed by the Kruskal-Wallis test, repeated-measures ANOVA or two-way ANOVA, and Tukey’s and Dunn’s post-hoc tests (α=0.05). The agar diffusion test showed a higher antibacterial property for 5% n-TiO2 compared with 3% and 7% (p<0.05) with no effect of time (1, 3, and 7 days). The cell number was significantly affected by all n-TiO2 groups, while viability was mostly affected by 3% and 5% n-TiO2, which also affected cell morphology and organization. Real-time PCR demonstrated that n-TiO2 reduced the expression of covR when compared with GIC with no n-TiO2 (p<0.05), with no effect of time, except for 3% n-TiO2 on vicR expression. Within-group and between-group analyses revealed n-TiO2 did not affect mRNA levels of gtfB, gtfC, and gtfD (p>0.05). Incorporation of n-TiO2 at 3% and 5% potentially affected S. mutans viability and the expression of key genes for bacterial survival and growth, improving the anticariogenic properties of GIC. (AU)

• Processo FAPESP: 16/13786-0 - Influência de Nova Nanotecnologia nas Propriedades Físico-Químicas-Biológicas do Cimento de Ionômero de Vidro Convencional
• Beneficiário: Kamila Rosamilia Kantovitz
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/14078-8 - Avaliação do efeito da incorporação de nanotubos de dióxido de titânio ao cimento de ionômero de vidro convencional sobre suas propriedades biológicas
• Beneficiário: Kamila Rosamilia Kantovitz
• Modalidade de apoio: Auxílio à Pesquisa - Regular