Probiotics improve re-epithelialization of scratches infected by Porphyromonas gingivalis through up-regulating CXCL8-CXCR1/CXCR2 axis

Autor(es)

Albuquerque-Souza, Emmanuel ^{[1, 2]} ; Ishikawa, Karin Hitomi ^[2] ; Amado, Pamela Penas ^[1] ; Nicoli, Jacques Robert ^[3] ; Holzhausen, Marinella ^[2] ; Mayer, Marcia P. A. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, SP - Brazil

^[2] Univ Sao Paulo, Dept Stomatol, Div Periodont, Sch Dent, Sao Paulo, SP - Brazil

^[3] Univ Fed Minas Gerais, Biol Sci Inst, Dept Microbiol, Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Anaerobe; v. 72, DEC 2021.
• Citações Web of Science: 0

Resumo

Porphyromonas gingivalis inhibits the release of CXCL8 by gingival epithelial cells and reduces their proliferation. We previously reported that Bifidocaterium sp. and Lactobacillus sp. immunomodulate gingival epithelial cells response to this periodontal pathogen, but their effects on re-epithelialization properties are still unknown. Herein we explored these activities of potential probiotics on gingival epithelial cells and clarified their mechanisms. The immortalized OBA-9 lineage was used to perform in vitro scratches. Twelve clinical isolates and commercially available strains of Bifidobacterium sp. and Lactobacillus sp. were screened. L. casei 324 m and B. pseudolongum 119(1A) were selected to perform mechanistic assays with P. gingivalis W83 infection and the following parameters were measured: percentage of re-epithelialization by DAPI immunofluorescence area measurement; cell number by Trypan Blue exclusion assay; CXCL8 regulation by ELISA and RT-qPCR; and expression of CXCL8 cognate receptors-CXCR1 and CXCR2 by Flow Cytometry. Complementary mechanistic assays were performed with CXCL8, in the presence or absence of the CXCR1/CXCR2 inhibitor-reparixin. L. casei 324 m and B. pseudolongum 119(1A) enhanced re-epithelialization/cell proliferation as well as inhibited the harmful effects of P. gingivalis W83 on these activities through an increase in the expression and release of CXCL8 and in the number of cells positive for CXCR1/CXCR2. Further, we revealed that the beneficial effects of these potential probiotics were dependent on activation of the CXCL8-CXCR1/CXCR2 axis. The current findings indicate that these potential probiotics strains may improve wound healing in the context of the periodontal tissues by a CXCL8 dependent mechanism. (C) 2021 Published by Elsevier Ltd. (AU)

• Processo FAPESP: 16/13156-7 - Efeitos direto e imunomodulatório das bactérias probióticas sobre os patógenos periodontais
• Beneficiário: Karin Hitomi Ishikawa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/18273-9 - Novas estratégias para o controle das periodontites
• Beneficiário: Marcia Pinto Alves Mayer
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/14687-6 - Avaliação do potencial imunomodulador de probióticos na doença periodontal
• Beneficiário: Emmanuel Albuquerque de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado