Colonization and local host response following intramammary Staphylococcus chromogenes challenge in dry cows

Autor(es)

Beuckelaere, Lisa ^[1] ; De Visscher, Anneleen ^{[1, 2]} ; Souza, Fernando Nogueira ^{[3, 4]} ; Meyer, Evelyne ^[5] ; Haesebrouck, Freddy ^[6] ; Piepers, Sofie ^[1] ; De Vliegher, Sarne ^[1]

Afiliação do(s) autor(es)

^[1] Univ Ghent, M Team & Mastitis & Milk Qual Res Unit, Dept Internal Med Reprod & Populat Med, Fac Vet Med, Salisburylaan 133, B-9820 Merelbeke - Belgium

^[2] Flanders Res Inst Agr Fisheries & Food ILVO, Technol & Food Sci, Burgemeester Van Gansberghelaan 115 Bus 1, B-9820 Merelbeke - Belgium

^[3] Univ Sao Paulo, Vet Clin Immunol Res Grp, Dept Clin Med, Fac Med Vet & Zootecnia, Av Prof Dr Orlando Marques Paiva 87, BR-05508270 Sao Paulo - Brazil

^[4] Univ Fed Paraiba, Programa Posgrad Ciencia Anim, Ctr Ciencias Agr, BR-58397000 Areia - Brazil

^[5] Univ Ghent, Fac Vet Med, Dept Vet & Biosci, Salisburylaan 133, B-9820 Merelbeke - Belgium

^[6] Univ Ghent, Fac Vet Med, Dept Pathobiol Pharmacol & Zool Med, Salisburylaan 133, B-9820 Merelbeke - Belgium

• Tipo de documento: Artigo Científico
• Fonte: VETERINARY RESEARCH; v. 52, n. 1 OCT 28 2021.
• Citações Web of Science: 0

Resumo

Although extensive research has been performed on bovine non-aureus staphylococci (NAS), several aspects such as bacteria-host interaction remain largely unstudied. Moreover, only a few mastitis pathogen challenge studies in cows have been conducted in the dry period, an important period that allows intramammary infection (IMI) to cure and new IMI to occur. We challenged 16 quarters of 4 Holstein Friesian cows at dry off with 100; 100 000 or 10 000 000 CFU of the udder-adapted S. chromogenes IM strain. Four quarters from one cow served as negative controls. Internally sealed quarters remained untouched, whereas non-sealed quarters were sampled 3 times during the dry period. After parturition, colostrum and daily milk samples were taken during the first week of lactation of all quarters. In total, 8 quarters appeared to be colonized, since S. chromogenes IM was recovered at least once during the experiment, as substantiated using Multilocus Sequence Typing. S. chromogenes IM shedding was highest in dry quarters inoculated with 10 000 000 CFU. Colonized quarters had the highest quarter somatic cell count (qSCC) in early lactation. Inoculated quarters (both colonized and non-colonized) had lower IL-6 and IL-10 concentrations in the dry period, whilst IFN-y levels tended to be higher in colonized quarters compared to non-inoculated quarters. Also, IgG2 levels were higher in inoculated compared to non-inoculated quarters and the IgG2/IgG1 ratio was on average above 1.To conclude, we showed that dry quarters can be colonized with S. chromogenes IM, resulting in a shift towards a Th1 response in late gestation and early lactation characterised by an increased IgG2 concentration. However, further research is needed to confirm our findings. (AU)

• Processo FAPESP: 17/07921-5 - Interação das células epiteliais mamárias com as células mononucleares do sangue periférico na infecção intramamária por staphylococcus aureus e distintas espécies e estirpes de estafilococos coagulase-negativos: desenvolvimento de um modelo in vitro
• Beneficiário: Fernando Nogueira de Souza
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 14/23189-4 - Interação das células epiteliais mamárias e das células mononucleares do sangue periférico na regulação da resposta imune e na infecção intramamária por Staphylococcus aureus, em bovinos
• Beneficiário: Fernando Nogueira de Souza
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado