Hepatitis B in the Northwestern region of Sao Paulo State: genotypes and resistance mutations

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Brígida Helena da Silva Meneghello ^[1] ; Márcia Maria Costa Nunes Soares ^[2] ; Vanessa Cristina Martins Silva ^[3] ; Marcilio Figueiredo Lemos ^[4] ; Murilo Castro Cervato ^[5] ; João Caetano Filho ^[6] ; Roberta Sitnik ^[7] ; Tânia Cristina Higino Estécio ^[8] ; Adriana Parise Compri ^[9] ; João Renato Rebello Pinho ; Regina Célia Moreira ^[11]

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^[1] Instituto Adolfo Lutz. Centro de Laboratório Regional - Brasil

^[2] Instituto Adolfo Lutz. Centro de Laboratório Regional - Brasil

^[3] Instituto Adolfo Lutz. Laboratório de Hepatites - Brasil

^[4] Instituto Adolfo Lutz. Laboratório de Hepatites - Brasil

^[5] Hospital Israelita Albert Einstein - Brasil

^[6] Instituto Adolfo Lutz. Centro de Laboratório Regional - Brasil

^[7] Hospital Israelita Albert Einstein - Brasil

^[8] Instituto Adolfo Lutz. Centro de Laboratório Regional - Brasil

^[9] Instituto Adolfo Lutz. Laboratório de Hepatites - Brasil

^[11] Instituto Adolfo Lutz. Laboratório de Hepatites - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Revista do Instituto de Medicina Tropical de São Paulo; v. 63, 2021-11-08.

Resumo

ABSTRACT In Brazil, few studies on the molecular aspects of hepatitis B virus (HBV) infection have been conducted in the interior regions of Sao Paulo State. This study aimed to identify HBV genotypes and evaluate strains with resistance mutations for nucleoside analogues in the Administrative Region (AR) of the municipality of Sao Jose do Rio Preto. We performed nested PCRs of 127 samples from the Health Care Services of the AR to amplify, sequence and analyze fragments of the HBV DNA, in order to identify genotypes and resistance mutations. The HBV S/Pol regions of 126 samples were successfully amplified and sequenced. Five different genotypes were found, and the main ones were A, D and F; a greater number of samples contained the subgenotypes A1 (n = 51; 40.5%), D3 (n = 36; 28.6%), A2 (n = 14; 11.1%) and F2a (n = 9; 7.1%). Resistance mutations (rtM204V/I/S) associated or not with compensatory mutations (rtL180M, rtV173L) were identified in 13.9% (5/36) of patients undergoing viral treatment and 1.1% (1/90) of naïve patients. The diversity of genotypes/subgenotypes found is probably due to the intense migration occurring in the region. These data can complement epidemiological and clinical surveillance, and can be used for a more effective management of chronic HBV patients. (AU)

• Processo FAPESP: 17/01809-9 - Caracterização de mutações de resistência primária para as drogas antivirais de ação direta (DAA) para o vírus da Hepatite C
• Beneficiário: Regina Célia Moreira
• Modalidade de apoio: Auxílio à Pesquisa - Regular