Capacitive electrical stimulation of a conducting polymeric thin film induces human mesenchymal stem cell osteogenesis

Autor(es)

da Silva, Rubens Araujo ^[1] ; Xue, Ruikang ^{[2, 3]} ; de Torresi, Susana Ines Cordoba ^[1] ; Cartmell, Sarah ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Av Prof Lineu Prestes 748, CP 26077, BR-05508000 Sao Paulo - Brazil

^[2] Univ Manchester, Fac Sci & Engn, Sch Nat Sci, Dept Mat, Royce Hub Bldg, Manchester M13 9PL, Lancs - England

^[3] Univ Manchester, Henry Royce Inst, Royce Hub Bldg, Manchester M13 9PL, Lancs - England

• Tipo de documento: Artigo Científico
• Fonte: BIOINTERPHASES; v. 17, n. 1 JAN 2022.
• Citações Web of Science: 0

Resumo

Electroactive materials based on conductive polymers are promising options for tissue engineering and regenerative medicine applications. In the present work, the conducting copolymers of poly (3,4-ethylenedioxythiophene) and poly (d, l-lactic acid) (PEDOT-co-PDLLA) with PEDOT:PDLLA molar ratios of 1:50, 1:25, and 1:5 were synthesized and compared to the insulating macromonomer of EDOT-PDLLA as an experimental control. Bone marrow-derived human mesenchymal stem cells (hMSC-BM) were cultured on the copolymers and the macromonomer thin films inside a bioreactor that induced a capacitive electrical stimulation (CES) with an electric field of 100 mV/mm for 2 h per day for 21 days. Under CES, the copolymers exhibited good cell viability and promoted the differentiation from hMSC-BM to osteogenic lineages, revealed by higher mineralization mainly when the contents of conducting segments of PEDOT (i.e., copolymer with 1:25 and 1:5 PEDOT:PDLLA ratios) were increased. The results indicate that the intrinsic electrical conductivity of the substrates is an important key point for the effectiveness of the electric field generated by the CES, intending to promote the differentiation effect for bone cells. (AU)

• Processo FAPESP: 15/26308-7 - Otimização das propriedades físico-químicas de materiais nano-estruturados e suas aplicações em reconhecimento molecular, catálise e conversão/armazenamento de energia
• Beneficiário: Roberto Manuel Torresi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/18846-7 - Avaliação do processo de adesão e proliferação celular em filmes finos de PEDOT-co-PDLLA sob regime de estímulo elétrico e aplicação de força mecânica
• Beneficiário: Rubens Araujo da Silva
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 17/08349-3 - Estudo do processo de adesão e proliferação celular em filmes finos de PEDOT-co-PDLLA suportado em eletrodo de Au funcionalizado com laminina, integrina e fibronectina
• Beneficiário: Rubens Araujo da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado