Protective Immunity against Gamma and Zeta Variants after Inactivated SARS-CoV-2 Virus Immunization

Autor(es):Mostrar menos -

Fumagalli, Marcilio Jorge ^{[1, 2]} ; Castro-Jorge, Luiza Antunes ^[1] ; Fraga-Silva, Thais Fernanda de Campos ^[3] ; de Azevedo, Patrick Orestes ^[4] ; Capato, Carlos Fabiano ^[1] ; Rattis, Bruna Amanda Cruz ^[5] ; Hojo-Souza, Natalia Satchiko ^[4] ; Floriano, Vitor Goncalves ^[1] ; de Castro, Julia Teixeira ^[4] ; Ramos, Simone Gusmao ^[5] ; da Fonseca, Benedito Antonio Lopes ^[1] ; Bonato, Vania Luiza Deperon ^{[3, 2]} ; Gazzinelli, Ricardo Tostes ^{[2, 4, 6]} ; Figueiredo, Luiz Tadeu Moraes ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Virol Res Ctr, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Basic & Appl Immunol Program, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, BR-14049900 Ribeirao Preto, SP - Brazil

^[4] Fundacao Oswaldo Cruz, Rene Rachou Inst, Immunopathol Lab, BR-30190002 Belo Horizonte, MG - Brazil

^[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pathol, BR-14049900 Ribeirao Preto, SP - Brazil

^[6] Univ Sao Paulo, Ribeirao Preto Med Sch, Platform Translat Med, Fundacao Oswaldo Cruz, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Viruses-Basel; v. 13, n. 12 DEC 2021.
• Citações Web of Science: 0

Resumo

The persistent circulation of SARS-CoV-2 represents an ongoing global threat due to the emergence of new viral variants that can sometimes evade the immune system of previously exposed or vaccinated individuals. We conducted a follow-up study of adult individuals that had received an inactivated SARS-CoV-2 vaccine, evaluating antibody production and neutralizing activity over a period of 6 months. In addition, we performed mice immunization with inactivated SARS-CoV-2, and evaluated the immune response and pathological outcomes against Gamma and Zeta variant infection. Vaccinated individuals produced high levels of antibodies with robust neutralizing activity, which was significantly reduced against Gamma and Zeta variants. Production of IgG anti-S antibodies and neutralizing activity robustly reduced after 6 months of vaccination. Immunized mice demonstrated cellular response against Gamma and Zeta variants, and after viral infection, reduced viral loads, IL-6 expression, and histopathological outcome in the lungs. TNF levels were unchanged in immunized or not immunized mice after infection with the Gamma variant. Furthermore, serum neutralization activity rapidly increases after infection with the Gamma and Zeta variants. Our data suggest that immunization with inactivated WT SARS-CoV-2 induces a promptly responsive cross-reactive immunity response against the Gamma and Zeta variants, reducing COVID-19 pathological outcomes. (AU)

• Processo FAPESP: 19/26119-0 - Vírus emergentes e reemergentes: biologia, patogênese e prospecção
• Beneficiário: Eurico de Arruda Neto
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 20/05527-0 - Vacina intranasal bivalente utilizando vírus influenza expressando a proteína S (spike) do SARS-CoV-2: mecanismos de proteção e lesão pulmonar
• Beneficiário: Ricardo Tostes Gazzinelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/09383-3 - Estudo sobre epitopos de Flavivirus com importância médica no Brasil e de seus anticorpos
• Beneficiário: Marcilio Jorge Fumagalli
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/09881-6 - Papel da sinalização dependente de TLR2 e TLR9 nas células epiteliais ou dendríticas na inflamação pulmonar durante a reexposição ao alérgeno e a infecção com pneumococo
• Beneficiário: Thais Fernanda de Campos Fraga da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/11213-1 - EMU concedido no processo 2017/21629-5, citômetro FACS Melody 2-lasers, 6-colors (4-2) blue violet
• Beneficiário: Vânia Luiza Deperon Bonato
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários