Carvalho, Bruna G.
Garcia, Bianca B. M.
Malfatti-Gasperini, Antonio A.
Han, Sang W.
de la Torre, Lucimara G.
Número total de Autores: 5
Afiliação do(s) autor(es):
 Univ Estadual Campinas, Sch Chem Engn, Dept Mat & Bioproc Engn, UNICAMP, BR-13083970 Campinas - Brazil
 Fed Univ Sao Paulo UNIFESP, Ctr Cell Therapy & Mol, BR-04044010 Sao Paulo - Brazil
 Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Synchrotron Light Lab LNLS, BR-13083970 Campinas, SP - Brazil
Número total de Afiliações: 3
Tipo de documento:
COLLOIDS AND SURFACES B-BIOINTERFACES;
Citações Web of Science:
The association of cationic carriers with different anionic mucoadhesive biopolymers has been widely explored as an alternative to improve their delivery routes and specific targeting. This work presents a complete analysis of the association between chondroitin sulfate (CS) and cationic liposomes (CLs)/lipoplex (CL-pDNA). In this study, plasmid DNA (pDNA) was used as a genetic cargo for association with carriers. Firstly, we measured the stoichiometry of pseudo complexes and evaluated their colloidal properties, structural and morphological characteristics. Optimized CL-pDNA lipoplexes (positive z-potential) and CL-CS / CL-pDNA-CS (negative z-potential with CS mass ratio of 9% (w/w)) were further studied in detail. Small-angle X-ray scattering analysis and cryo-transmission electron microscopy micrographs revealed that the electrostatic interaction between CS and CL / CL-pDNA easily reorganized the lipid bilayers resulting in nanoscale uni/multilamellar vesicles. A high CS mass ratio (9% (w/w)) led to the reassembly of liposomal structure, wherein the pDNA was easily exchanged for CS chains, forming more than 50% of dense multilamellar vesicles. This data evidenced that the association between CS and CLs is not a conventional coating process since it generates complex and hybrid structures. We believe that these obtained colloidal data may be used in the future to investigate polymer-tailored nanocarriers and their production process. In brief, the colloidal study of hybrid structures may open interesting perspectives for developing novel carriers for drug and gene delivery applications. (AU)