Interaction of periodontitis and orthodontic tooth movement-an in vitro and in vivo study

Autor(es)

Rath-Deschner, Birgit ^[1] ; Nogueira, Andressa V. B. ^[2] ; Beisel-Memmert, Svenja ^[1] ; Nokhbehsaim, Marjan ^[3] ; Eick, Sigrun ^[4] ; Cirelli, Joni A. ^[5] ; Deschner, James ^[2] ; Jaeger, Andreas ^[1] ; Damanaki, Anna ^[2]

Afiliação do(s) autor(es)

^[1] Univ Bonn, Ctr Dentomaxillofacial Med, Dept Orthodont, Welschnonnenstr 17, D-53111 Bonn - Germany

^[2] Johannes Gutenberg Univ Mainz, Dept Periodontol & Operat Dent, Univ Med Ctr, Mainz - Germany

^[3] Univ Bonn, Ctr Dentomaxillofacial Med, Sect Expt Dentomaxillofacial Med, Bonn - Germany

^[4] Univ Bern, Dept Periodontol, Lab Oral Microbiol, Bern - Switzerland

^[5] Sao Paulo State Univ, Sch Dent Araraquara, Dept Diag & Surg, UNESP, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 26, n. 1, p. 171-181, JAN 2022.
• Citações Web of Science: 1

Resumo

Objectives The aim of this in vitro and in vivo study was to investigate the interaction of periodontitis and orthodontic tooth movement on interleukin (IL)-6 and C-X-C motif chemokine 2 (CXCL2). Materials and methods The effect of periodontitis and/or orthodontic tooth movement (OTM) on alveolar bone and gingival IL-6 and CXCL2 expressions was studied in rats by histology and RT-PCR, respectively. The animals were assigned to four groups (control, periodontitis, OTM, and combination of periodontitis and OTM). The IL-6 and CXCL2 levels were also studied in human gingival biopsies from periodontally healthy and periodontitis subjects by RT-PCR and immunohistochemistry. Additionally, the synthesis of IL-6 and CXCL2 in response to the periodontopathogen Fusobacterium nucleatum and/or mechanical strain was studied in periodontal fibroblasts by RT-PCR and ELISA. Results Periodontitis caused an increase in gingival levels of IL-6 and CXCL2 in the animal model. Moreover, orthodontic tooth movement further enhanced the bacteria-induced periodontal destruction and gingival IL-6 gene expression. Elevated IL-6 and CXCL2 gingival levels were also found in human periodontitis. Furthermore, mechanical strain increased the stimulatory effect of F. nucleatum on IL-6 protein in vitro. Conclusions Our study suggests that orthodontic tooth movement can enhance bacteria-induced periodontal inflammation and thus destruction and that IL-6 may play a pivotal role in this process. (AU)

• Processo FAPESP: 14/20715-7 - Influência do movimento ortodôntico na progressão da doença periodontal em condições de saúde sistêmica ou obesidade
• Beneficiário: Andressa Vilas Boas Nogueira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/07137-2 - Efeitos da obesidade nos tecidos periodontais sujeitos a forças biomecânicas
• Beneficiário: Joni Augusto Cirelli
• Modalidade de apoio: Auxílio à Pesquisa - Regular