Distinct behavior of bovine-associated staphylococci species in their ability to resist phagocytosis and trigger respiratory burst activity by blood and milk polymorphonuclear leukocytes in dairy cow

Autor(es):Mostrar menos -

Souza, Rodrigo M. ^[1] ; Souza, Fernando N. ^{[2, 1, 3]} ; Batista, Camila F. ^[1] ; Piepers, Sofie ^[3] ; De Visscher, Anneleen ^[3] ; Santos, Kamila R. ^[1] ; Molinari, Paula C. ^{[4, 1]} ; Ferronatto, Jose A. ^[1] ; da Cunha, Adriano Franca ^[5] ; Blagitz, Maiara G. ^{[1, 6, 7]} ; da Silva, Guilherme G. ^[8] ; Renno, Francisco P. ^[8] ; Cerqueira, Monica M. O. P. ^[5] ; Heinemann, Marcos B. ^[9] ; De Vliegher, Sarne ^[3] ; Della Libera, Alice M. M. P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Clin Med, Vet Clin Immunol Res Grp, Av Prof Dr Orlando Marques de Paiva 87, BR-05508270 Sao Paulo - Brazil

^[2] Univ Fed Paraiba, Programa Posgrad Ciencia Anim, BR-58397000 Areia - Brazil

^[3] Univ Ghent, Fac Vet Med, M Team & Mastitis & Milk Qual Res Unit, Dept Reprod Obstet & Herd Hlth, Salisburylaan 133, B-9820 Merelbeke - Belgium

^[4] Univ Florida, Dept Anim Sci, Gainesville, FL 32611 - USA

^[5] Univ Fed Minas Gerais, Dept Tecnol & Inspecao Prod Origem Anim, Escola Vet, BR-31270010 Belo Horizonte, MG - Brazil

^[6] Univ Fed Fronteira Sul, Curso Med Vet, Campus Realeza, BR-85770000 Realeza - Brazil

^[7] Programa Posgrad Saude Bem Estar & Prod Anim Sust, Campus Realeza, BR-85770000 Realeza - Brazil

^[8] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Nutr & Prod Anim, BR-13635900 Pirassununga - Brazil

^[9] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Med Vet Prevent & Saude Anim, BR-05508270 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF DAIRY SCIENCE; v. 105, n. 2, p. 1625-1637, FEB 2022.
• Citações Web of Science: 0

Resumo

Mastitis affects a high proportion of dairy cows and is still one of the greatest challenges faced by the dairy industry. Staphylococcal bacteria remain the most important cause of mastitis worldwide. We investigated how distinct staphylococcal species evade some critical host defense mechanisms, which may dictate the establishment, severity, and persistence of infection and the outcome of possible therapeutic and prevention interventions. Thus, the present study investigated variations among distinct bovine-associated staphylococci in their capability to resist phagocytosis and to trigger respiratory burst activity of blood and milk polymorphonuclear neutrophil leukocytes (PMNL) in dairy cows. To do so, PMNL of 6 primiparous and 6 multiparous dairy cows were used. A collection of 38 non-aureus staphylococci (NAS) and 12 Staphylococcus aureus were included. The phagocytosis and intracellular reactive oxygen species (ROS) production by blood and milk PMNL were analyzed by flow cytometry. Phagocytosis, by both blood and milk PMNL, did not differ between S. aureus and NAS as a group, although within-NAS species differences were observed. Staphylococcus chromogenes (a so-called milk-adapted NAS species) better resisted phagocytosis by blood PMNL than the so-called environmental (i.e., Staphylococcus fleurettii) and opportunistic (i.e., Staphylococcus haemolyticus) NAS species. Otherwise, S. haemolyticus was better phagocytosed by blood PMNL than S. aureus, S. fleurettii, and S. chromogenes. No influence of the origin of the isolates within the staphylococci species in the resistance to phagocytosis by blood and milk PMNL was found. Overall, both S. aureus and NAS did not inhibit intracellular ROS production in blood and milk PMNL. Non-aureus staphylococci induced fewer ROS by milk PMNL than S. aureus, which was not true for blood PMNL, although species-specific differences in the intensity of ROS production were observed. Staphylococcus chromogenes induced more blood PMNL ROS than S. fleurettii and S. haemolyticus, and as much as S. aureus. Conversely, S. chromogenes induced fewer milk PMNL ROS than S. aureus. The origin of the isolates within the staphylococci species did not affect the ROS production by blood and milk PMNL. In conclusion, our study showed differences in staphylococci species in evading phagocytosis and triggering ROS production, which may explain the ability of some staphylococci species (i.e., S. aureus and S. chromogenes) to cause persistent infection and induce inflammation. (AU)

• Processo FAPESP: 16/06297-3 - Morte bacteriana e fagocitose por fagócitos lácteos de distintos isolados de estafilococos associados aos bovinos em vacas leiteiras primíparas e multíparas
• Beneficiário: Paula Cristina Cardoso Molinari
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
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• Beneficiário: Fernando Nogueira de Souza
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
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• Beneficiário: José Augusto Ferronatto
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 15/16871-6 - Morte bacteriana e fagocitose por neutrófilos de distintos isolados de estafilococos associados aos bovinos em vacas leiteiras primíparas e multíparas
• Beneficiário: Alice Maria Melville Paiva Della Libera
• Modalidade de apoio: Auxílio à Pesquisa - Regular
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• Beneficiário: Fernando Nogueira de Souza
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado