iagnostics of SARS-CoV-2 infection using electrical impedance spectroscopy with an immunosensor to detect the spike protei

Autor(es)

Soares, Juliana C. ^[1] ; Soares, Andrey C. ^[2] ; Angelim, Monara Kaelle S. C. ^[3] ; Proenca-Modena, Jose Luiz ^{[4, 3]} ; Moraes-Vieira, Pedro M. ^{[4, 3, 5]} ; Mattoso, Luiz H. C. ^[2] ; Oliveira Jr, Osvaldo N.

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sao Carlos Inst Phys IFSC, BR-13566590 Sao Carlos - Brazil

^[2] Embrapa Instrumentacao, Nanotechnol Natl Lab Agr LNNA, BR-13560970 Sao Carlos, SP - Brazil

^[3] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, BR-13083862 Campinas, SP - Brazil

^[4] Univ Estadual Campinas, Expt Med Res Cluster EMRC, BR-13083862 Campinas, SP - Brazil

^[5] Univ Estadual Campinas, Obes & Comorbil Res Ctr OCRC, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Talanta; v. 239, MAR 1 2022.
• Citações Web of Science: 1

Resumo

Mass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10-20 g/mL to 10-14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C-H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing even in places with limited resources. (AU)

• Processo FAPESP: 18/18953-8 - Filmes nanoestruturados aplicados em biossensores microfluídicos para detecção de mastite bacteriana
• Beneficiário: Andrey Coatrini Soares
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/22214-6 - Rumo à convergência de tecnologias: de sensores e biossensores à visualização de informação e aprendizado de máquina para análise de dados em diagnóstico clínico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 20/08744-2 - Estudo sobre fatores de risco associados à maior gravidade a COVID-19 e mapeamento de vias metabólicas necessárias para a resposta anti-SARS-CoV-2
• Beneficiário: Monara Kaélle Sérvulo Cruz Angelim
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 20/04579-7 - Estudo sobre fatores de risco associados à maior gravidade a COVID-19 e mapeamento de vias metabólicas necessárias para a resposta anti-SARS-CoV-2
• Beneficiário: Pedro Manoel Mendes de Moraes Vieira
• Modalidade de apoio: Auxílio à Pesquisa - Regular