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Extracellular matrix-derived and low-cost proteins to improve polyurethane-based scaffolds for vascular grafts

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Rodrigues, Isabella C. P. ; Lopes, Eder S. N. ; Pereira, Karina D. ; Huber, Stephany C. ; Jardini, Andre Luiz ; Annichino-Bizzacchi, Joyce M. ; Luchessi, Augusto D. ; Gabriel, Lais P.
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: SCIENTIFIC REPORTS; v. 12, n. 1, p. 12-pg., 2022-03-28.

Vascular graft surgeries are often conducted in trauma cases, which has increased the demand for scaffolds with good biocompatibility profiles. Biodegradable scaffolds resembling the extracellular matrix (ECM) of blood vessels are promising vascular graft materials. In the present study, polyurethane (PU) was blended with ECM proteins collagen and elastin (Col-El) and gelatin (Gel) to produce fibrous scaffolds by using the rotary jet spinning (RJS) technique, and their effects on in vitro properties were evaluated. Morphological and structural characterization of the scaffolds was performed using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Micrometric fibers with nanometric rugosity were obtained. Col-El and Gel reduced the mechanical strength and increased the hydrophilicity and degradation rates of PU. No platelet adhesion or activation was observed. The addition of proteins to the PU blend increased the viability, adhesion, and proliferation of human umbilical vein endothelial cells (HUVECs). Therefore, PU-Col-El and PU-Gel scaffolds are promising biomaterials for vascular graft applications. (AU)

Processo FAPESP: 20/07923-0 - Desenvolvimento de biotintas bioativas e produção de scaffolds bioabsorvíveis customizados por manufatura aditiva e rotofiação para o reparo de tecidos ósseos
Beneficiário:Laís Pellizzer Gabriel
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/13273-6 - Desenvolvimento de scaffolds tubulares para aplicações na engenharia de tecidos vascular
Beneficiário:Laís Pellizzer Gabriel
Modalidade de apoio: Auxílio à Pesquisa - Regular