Successful invasion of Trypanosoma cruzi trypomastigotes is dependent on host cell actin cytoskeleton

Autor(es)

Bonifacio, Bruno Souza ; Bonfim-Melo, Alexis ; Mortara, Renato Arruda ; Ferreira, Eden Ramalho

• Tipo de documento: Artigo Científico
• Fonte: Journal of Eukaryotic Microbiology; v. 69, n. 3, p. 10-pg., 2022-03-31.

Resumo

Cellular invasion by Trypanosoma cruzi metacyclic trypomastigotes (MTs) or tissue culture trypomastigotes (TCTs) is a complex process involving host-parasite cellular and molecular interactions. Particularly, the involvement of host cell actin cytoskeleton during trypomastigote invasion is poorly investigated, and still, the results are controversial. In the present work, we compare side by side both trypomastigote forms and employ state-of-the-art live-cell imaging showing for the first time the dynamic mobilization of host cell actin cytoskeleton to MT and TCT invasion sites. Moreover, cytochalasin D, latrunculin B, and jasplakinolide-pretreated cells inhibited MT and TCT invasion. Furthermore, our results demonstrated that TCT invasion decreased in RhoA, Rac1, and Cdc-42 GTPase-depleted cells, whereas MT invasion decreased only in Cdc42-and RhoA-depleted cells. Interestingly, depletion of the three studied GTPases induced a scattered lysosomal distribution throughout the cytosol. These observations indicate that GTPase depletion is sufficient to impair parasite invasion despite the importance of lysosome spread in trypomastigote invasion. Together, our results demonstrate that the host cell actin cytoskeleton plays a direct role during TCT and MT invasion. (AU)

• Processo FAPESP: 16/16918-5 - Estudo do egresso de tripomastigotas de Trypanosoma cruzi em células infectadas
• Beneficiário: Éden Ramalho de Araujo Ferreira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/24701-9 - Papel das GTPases Cdc42, RhoA e Rac1 no citoesqueleto de actina durante a invasão celular pelas formas tripomastigotas metacíclicas e de cultura de tecido de Trypanosoma cruzi em células HeLa
• Beneficiário: Bruno Souza Bonifácio
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/17770-1 - Identificação e caracterização de novos mediadores do citoesqueleto de actina durante a invasão celular pelas formas amastigotas extracelulares de Trypanosoma cruzi
• Beneficiário: Alexis de Sá Ribeiro do Bonfim de Melo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/15000-4 - Trypanosoma cruzi: variabilidade genômica intra- e interespecífica e mecanismos de invasão/evasão celular
• Beneficiário: Renato Arruda Mortara
• Modalidade de apoio: Auxílio à Pesquisa - Temático