Thalidomide measurement in plasma and dried plasma spot by SPE combined with UHPLC-MS/MS for therapeutic drug monitoring

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Novellino, Renata Soares ; Chalom, Marc Yves ; Romano, Paschoalina ; Rezende Ebner, Persio de Almeida ; Seraphim, Julia Celestino ; Silva do Amaral, Ana Carolina ; Coelho Duarte, Nilo Jose ; Pedrosa, Tatiana Do Nascimento ; Neves Yuki, Emily Figueiredo ; Aikawa, Nadia Emi ; Pasoto, Sandra Gofinet ; Almeida da Silva, Clovis Artur ; Carvalho, Valdemir Melechco ; Bonfa, Eloisa ; Kanda Kupa, Leonard de Vinci

• Tipo de documento: Artigo Científico
• Fonte: BIOANALYSIS; v. 14, n. 15, p. 12-pg., 2022-09-20.

Resumo

Tweetable abstract Thalidomide measurement in dried plasma spot by ultra-HPLC-MS/MS combined with online SPE was successfully validated and applied in therapeutic drug monitoring. #thalidomide #DPS #chromatography #mass spectrometry Aims: To validate an SPE-ultra-HPLC-MS/MS method for thalidomide (THD) measurement in dried plasma spot (DPS). Methods: Extraction included acetonitrile/water clean-up and online SPE. The LOD, LLOQ, linearity, precision, accuracy, recovery, matrix effect, process efficiency, carryover, stability, drug interference and dilution integrity were assessed. Results: The method was linear from 50 to 2000 ng/ml with a LOD of 20 ng/ml and LLOQ of 50 ng/ml. The coefficient of variation for precision was 0.4-7.9% for intra-assay and 1.3-8.9% for interassay and accuracy was 81.4-97.1%. Adequate matrix effect (100.6-107.0%), recovery (88.7-105.0%) and process efficiency (91.3-109.3%) were registered. DPS was stable for 14 days at room temperature and 45 degrees C and for 4 months at -80 degrees C. The method was applied to quantify THD in both wet plasma and DPS from patients with cutaneous lupus receiving THD treatment. The difference between THD wet plasma and DPS concentration was Conclusion: The method is suitable to quantify THD in DPS. (AU)

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• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
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• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
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• Modalidade de apoio: Auxílio à Pesquisa - Temático