Silver Nimesulide Complex in Bacterial Cellulose Membranes as an Innovative Therapeutic Method for Topical Treatment of Skin Squamous Cell Carcinoma

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Candido, Tuany Zambroti ; Ferraz de Paiva, Raphael Enoque ; Figueiredo, Mariana Cecchetto ; Coser, Lilian de Oliveira ; Lazarini Frajacomo, Silmara Cristina ; Abbehausen, Camilla ; Cardinalli, Izilda Aparecida ; Lustri, Wilton Rogerio ; Carvalho, Joao Ernesto ; Tasca Gois Ruiz, Ana Lucia ; Corbi, Pedro Paulo ; Passos Lima, Carmen Silvia

• Tipo de documento: Artigo Científico
• Fonte: PHARMACEUTICS; v. 14, n. 2, p. 18-pg., 2022-02-01.

Resumo

Oxidative stress and inflammation act on skin squamous cell carcinoma (SSCC) development and progression. Curative therapy for SSCC patients is mainly based on surgical resection, which can cause various sequelae. Silver ions have in vitro activities over tumor cells, while nimesulide has antioxidant and anti-inflammatory activities. This study aimed to evaluate the effects of a silver(I) complex with nimesulide (AgNMS) incorporated in a sustained release device based on bacterial cellulose membrane, named AgNMS@BCM, on topic SSCC treatment. The antiproliferative effect of AgNMS complex was evaluated in the SCC4, SCC15 and FaDu SCC lines. AgNMS complex activity on exposure of phosphatidylserine (PS) residues and multicaspase activation were evaluated on FaDu cells by flow cytometry. The AgNMS@BCM effects were evaluated in a SSCC model induced by 7,12-dimethylbenzanthracene/12-o-tetradecanoyl-phorbol-13-acetate (DMBA/TPA) in mice. Toxicity and tumor size were evaluated throughout the study. AgNMS complex showed antiproliferative activity in SCC15 and FaDu lines in low to moderate concentrations (67.3 mu M and 107.3 mu M, respectively), and induced multicaspase activation on FaDu cells. The AgNMS@BCM did not induce toxicity and reduced tumor size up to 100%. Thus, the application of AgNMS@BCM was effective and safe in SSCC treatment in mice, and can be seen as a potential and safe agent for topic treatment of SSCC in humans. (AU)

• Processo FAPESP: 16/07729-4 - Complexos metálicos de ouro, paládio e prata no tratamento do carcinoma de células escamosas de pele
• Beneficiário: Tuany Zambroti Cândido
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/12062-4 - Síntese, caracterização e estudos de interação com biomoléculas de complexos metálicos contendo ligantes biologicamente ativos: uma estratégia na preparação de novos agentes com atividades antibacterianas e antivirais
• Beneficiário: Pedro Paulo Corbi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/09833-0 - Dispositivos de liberação sustentada baseados em biocelulose/complexos metálicos com ligantes bioativos
• Beneficiário: Wilton Rogério Lustri
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/12719-0 - Combate à resitência bacteriana: complexos metálicos e a inibição de metalo-beta-lactamses
• Beneficiário: Camilla Abbehausen
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/12590-0 - Síntese de complexos metálicos com ligantes bioativos N,N ou N,O-coordenantes para utilização em dispositivos de liberação sustentada baseados em celulose bacteriana
• Beneficiário: Wilton Rogério Lustri
• Modalidade de apoio: Auxílio à Pesquisa - Regular