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Genetic mapping of a bioethanol yeast strain reveals new targets for hydroxymethylfurfural- and thermotolerance

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Bezerra de Mello, Fellipe da Silveira ; Venega Coradini, Alessandro Luis ; Carazzolle, Marcelo Falsarella ; Maneira, Carla ; Furlan, Monique ; Guimaraes Pereira, Goncalo Amarante ; Teixeira, Gleidson Silva
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: MICROBIOLOGICAL RESEARCH; v. 263, p. 13-pg., 2022-08-02.

Current technology that enables bioethanol production from agricultural biomass imposes harsh conditions for Saccharomyces cerevisiae's metabolism. In this work, the genetic architecture of industrial bioethanol yeast strain SA-1 was evaluated. SA-1 segregant FMY097 was previously described as highly aldehyde resistant and here also as thermotolerant: two important traits for the second-generation industry. A Quantitative Trait Loci (QTL) mapping of 5-hydroxymethylfurfural (HMF) -resistant segregants of hybrid FMY097/BY4742 disclosed a region in chromosome II bearing alleles with uncommon non-synonymous (NS) single nucleotide polymorphisms (SNPs) in FMY097: MIX23, PKC1, SEA4, and SRO77. Allele swap to susceptible laboratory strain BY4742 revealed that SEA4(FMY097) enhances robustness towards HMF, but the industrial fitness could not be fully recovered. The genetic network arising from the causative genes in the QTL window suggests that intracellular signaling TOR (Target of Rapamycin) and CWI (Cell Wall Integrity) pathways are regulators of this phenotype in FMY097. Because the QTL mapping did not result in one major allelic contribution to the evaluated trait, a background effect in FMY097's HMF resistance is expected. Quantification of NADPH - cofactor implied in endogenous aldehyde detoxification reactions - supports the former hypothesis, given its high availability in FMY097. Regarding thermotolerance, SEA4(FMY097) grants BY4742 ability to grow in temperatures as high as 38 degrees C in liquid, while allele PKC1(FMY097) allows growth up to 40 degrees C in solid medium. Both SEA4(FMY097) and PKC1(FMY097) encode rare NS SNPs, not found in other > 1013S. cerevisiae. Altogether, these findings point towards crucial membrane and stress mediators for yeast robustness. (AU)

Processo FAPESP: 16/12852-0 - Implementação de um método de dissecção de tétrades de Saccharomyces cerevisiae baseado em citometria de fluxo
Beneficiário:Monique Furlan
Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
Processo FAPESP: 15/06677-8 - Engenharia metabólica e identificação de QTLs relacionados à tolerância ao HMF em Saccharomyces cerevisiae
Beneficiário:Fellipe da Silveira Bezerra de Mello
Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 13/08293-7 - CECC - Centro de Engenharia e Ciências Computacionais
Beneficiário:Munir Salomao Skaf
Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 16/02506-7 - Desenvolvimento de métodos high-throughput para o estudo de quantitative traits loci relacionados à robustez de leveduras industriais (Saccharomyces cerevisiae)
Beneficiário:Gleidson Silva Teixeira
Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
Processo FAPESP: 14/26719-4 - Mapeamento e estudo de QTLs relacionados à robustez da levedura industrial Pedra-2 (Saccharomyces cerevisiae)
Beneficiário:Alessandro Luis Venega Coradini
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 18/03403-2 - Engenharia metabólica de linhagens selvagens de Saccharomyces cerevisiae utilizando o sistema CRISPR/Cas9
Beneficiário:Carla Maneira da Silva
Modalidade de apoio: Bolsas no Brasil - Mestrado