Autor(es):Mostrar menos -

Castoldi, Robson Chacon ; Giometti, Ines Cristina ; Ozaki, Guilherme Akio Tamura ; Garcia, Thiago Alves ; Pereira, Joao Domingos Augusto dos Santos ; Rubira, Rafael Jesus Goncalves ; Constantino, Carlos Jose Leopoldo ; Junior, Silvio Assis de Oliveira ; Louzada, Mario Jefferson Quirino ; Belangero, William Dias ; Filho, Jose Carlos Silva Camargo

• Tipo de documento: Artigo Científico
• Fonte: VIBRATIONAL SPECTROSCOPY; v. 124, p. 8-pg., 2022-12-27.

Resumo

Purpose: To investigate the effects of applying growth hormone (GH) and performing a strength training protocol on mineralization, rigidity, and crystallinity on the bone tissue of Wistar rats.Method: In total, 40 male animals, 60 days old, of the Wistar lineage were used, distributed into four groups: control (C) [n = 10], control and application of GH (GHC) [n = 10], resistance training (T) [n = 10], and resistance training and application of GH (GHT) [n = 10]. The training period lasted four weeks and consisted of four sets of 10 jumps in a liquid medium. The amount of 0.2 IU per kilogram of body mass (0.2 IU/Kg) of GH was administered in the GHC and GHT groups. After the experimental period, samples of the tibia were collected and analyzed using Raman Spectroscopy (633 nm), Bone Densitometry -dual-energy x-ray absorptiometry (DXA), and Mechanical Assay (F-max).Results: The GHT group presented an increase for the variable Amide II (1544 cm-1) and decrease for the variable Proline (727 cm-1) from the Raman Spectroscopy analysis (p < 0.05). In addition, an increase was observed for the variables bone mineral density (BMD) and bone mineral content (BMC) (p < 0.05). Finally, no statistically significant differences were observed for the variables mineralization, crystallinity, phosphate carbonate types A and B, and bone rigidity (p > 0.05).Conclusion: It is concluded that GH added to RT resulted in an increase in the mineral Amide II (1544 cm-1) and a decrease in Proline (727 cm-1). Furthermore, an increase in BMD and BMC was observed only in the GHT group. Finally, no alterations were observed in crystallinity and rigidity in the different groups of animals. (AU)

• Processo FAPESP: 20/05423-0 - Detecção de agrotóxicos (coquetel) utilizados nas lavouras de cana-de-açúcar e o efeito destes sobre sistemas biológicos
• Beneficiário: Rafael Jesus Gonçalves Rubira
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/22214-6 - Rumo à convergência de tecnologias: de sensores e biossensores à visualização de informação e aprendizado de máquina para análise de dados em diagnóstico clínico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático