Antibiofilm Activity of LL-37 Peptide and D-Amino Acids Associated with Antibiotics Used in Regenerative Endodontics on an Ex Vivo Multispecies Biofilm Model

Autor(es)

Pereira, Ana C. C. ; Aguiar, Alana P. S. ; Araujo, Leticia M. P. ; Dantas, Larissa O. ; Mayer, Marcia P. A. ; Karygianni, Lamprini ; Thurnheer, Thomas ; Pinheiro, Ericka T.

• Tipo de documento: Artigo Científico
• Fonte: LIFE-BASEL; v. 12, n. 11, p. 8-pg., 2022-11-01.

Resumo

The antimicrobial peptide LL-37 and D-amino acids (D-AAs) have been proposed as antibiofilm agents. Therefore, this study aimed to test the antimicrobial effect of antibiofilm agents associated with antibiotics used in regenerative endodontic procedures (the triple antibiotic paste-TAP: ciprofloxacin + metronidazole + minocycline). An endodontic-like biofilm model grown on bovine dentin discs was used in this study. After 21-day growth, the biofilms were treated with 1 mg/mL TAP, 10 mu M LL-37, an association of LL-37 + TAP, 40 mM D-AAs solution, an association of D-AAs + TAP, and phosphate-buffered saline (negative control). Colony forming unit (CFU) data were analyzed by two-way ANOVA and Tukey's multiple comparison test (p < 0.05). LL-37 + TAP showed the best antibacterial activity (7-log(10) CFU/mL +/- 0.5), reaching a 1 log reduction of cells in relation to the negative control (8-log(10) CFU/mL +/- 0.7) (p < 0.05). In turn, no significant reduction in bacterial cells was observed with TAP, LL-37, D-AAs, and D-AAs + TAP compared to the negative control. In conclusion, the combination of antibiotics and LL-37 peptide showed mild antibacterial activity, while the combination of antibiotics and D-AAs showed no activity against complex biofilms. (AU)

• Processo FAPESP: 19/12908-3 - Modelos de biofilmes multiespécies para o estudo de novas estratégias de tratamento das infecções endodônticas persistentes/secundárias
• Beneficiário: Ericka Tavares Pinheiro
• Modalidade de apoio: Bolsas no Exterior - Pesquisa