Preventive effect of chitosan gel containing CaneCPI-5 against enamel erosive wear in situ

Autor(es):Mostrar menos -

Pela, Vinicius Taioqui ; Brito, Leonardo ; Taira, Even Akemi ; Henrique-Silva, Flavio ; Pieretti, Joana Claudio ; Seabra, Amedea Barozzi ; Baldini Cardoso, Cristiane de Almeida ; de Souza, Eduardo Pereira ; Groisman, Sonia ; Rodrigues, Marcela Charantola ; Lussi, Adrian ; Carvalho, Thiago Saads ; Rabelo Buzalaf, Marilia Afonso

• Tipo de documento: Artigo Científico
• Fonte: CLINICAL ORAL INVESTIGATIONS; v. 26, n. 11, p. 9-pg., 2022-07-13.

Resumo

Objective This study evaluated the preventive effect of a chitosan gel containing CaneCPI-5 against enamel erosion and erosion + abrasion in situ. Methods Sixteen volunteers participated in a crossover, double-blind protocol, comprising 4 phases: (1) no treatment (Nt); (2) chitosan gel (Cg); (3) chitosan gel + 12,300 ppm NaF (Cg + NaF); and (4) chitosan gel + 0.1 mg/mL CaneCPI-5 (Cg + Cane). Volunteers wore an appliance containing 4 specimens. Once/day, they applied the gel (except for Nt) (4 min/specimen). Erosive challenges were performed extra-orally (0.1% citric acid, 90 s, 4 x /day; ERO). Specimens were also abraded (toothbrush, 15 s/specimen, 2 x /day; ERO + ABR). Enamel wear was assessed by profilometry and relative surface reflection intensity (%SRI). Two-way RM-ANOVA/Sidak's tests and Spearman's correlation were used (p < 0.05). Results For profilometry, ERO + ABR promoted significantly greater wear when compared with ERO. There was a significant difference among all treatments. The lowest enamel loss occurred for Cg + Cane, followed by Cg + NaF, Cg, and Nt (p < 0.05). The %SRI was significantly lower for ERO + ABR when compared to ERO, only for the Nt group. The greatest %SRI was found for the Cg + NaF and Cg + Cane groups, which did not differ significantly, regardless of the conditions. The lowest %SRI was found for the Nt and Cg groups, which did not differ from each other, regardless of the conditions. The Nt group did not differ significantly from the Cg + NaF (ERO). There was a significant correlation between both analyses. Conclusion The incorporation of CaneCPI-5 in the chitosan gel prevented erosive wear in situ. (AU)

• Processo FAPESP: 20/03646-2 - Impacto das nanoplataformas baseadas em óxido nítrico e quimioterápicos na citotoxicidade e sensibilização de células tumorais resistentes
• Beneficiário: Joana Claudio Pieretti
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/08194-2 - Óleo essencial contendo nanopartículas metálicas funcionalizadas com óxido nítrico como estratégia para o controle de patógenos vegetais na agricultura
• Beneficiário: Amedea Barozzi Seabra
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/04857-4 - Engenharia de película adquirida para o controle da erosão dentária: avaliação in situ do potencial protetor de uma nova cistatina derivada da cana-de-açúcar
• Beneficiário: Vinícius Taioqui Pelá
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/12041-7 - Segurança e eficácia para prevenção da erosão dentária de uma nova cistatina derivada da cana-de-açúcar (CaneCPI-5): estudos in vitro, in situ e in vivo
• Beneficiário: Marília Afonso Rabelo Buzalaf
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 21/02366-9 - Engenharia de película adquirida com CaneCPI-5 adicionada a um gel de aplicação tópica contra a erosão dentinária: estudo in situ
• Beneficiário: Leonardo Brito
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica