Cytotoxicity towards Breast Cancer Cells of Pluronic F-127/Hyaluronic Acid Hydrogel Containing Nitric Oxide Donor and Silica Nanoparticles Loaded with Cisplatin

Autor(es)

Santana, Bianca de Melo ; Pieretti, Joana Claudio ; Gomes, Rafael Nunes ; Cerchiaro, Giselle ; Seabra, Amedea Barozzi

• Tipo de documento: Artigo Científico
• Fonte: PHARMACEUTICS; v. 14, n. 12, p. 16-pg., 2022-12-01.

Resumo

The incorporation of both nitric oxide (NO) donor (S-nitrosoglutathione, GSNO) and silica nanoparticles loaded with cisplatin (SiO2@CisPt NPs) into a polymeric matrix represents a suitable approach to creating a drug-delivery system with sustained and localized drug release against tumor cells. Herein, we report the synthesis, characterization, and cytotoxicity evaluation of Pluronic F-127/hyaluronic acid hydrogel containing GSNO and SiO2@CisPt NPs against breast cancer cells. SiO2@CisPt NPs were successfully synthesized, revealing a spherical morphology with an average size of 158 +/- 20 nm. Both GSNO and SiO2@CisPt NPs were incorporated into the thermoresponsive Pluronic/hyaluronic hydrogel for sustained and localized release of both NO and cisplatin. The kinetics of NO release from a hydrogel matrix revealed spontaneous and sustained release of NO at the millimolar range for 24 h. The MTT assay showed concentration-dependent cytotoxicity of the hydrogel. The combination of GSNO and SiO2@CisPt incorporated into a polymeric matrix decreased the cell viability 20% more than the hydrogel containing only GSNO or SiO2@CisPt. At 200 mu g/mL, this combination led to a critical cell viability of 30%, indicating a synergistic effect between GSNO and SiO2@CisPt NPs in the hydrogel matrix, and, therefore, highlighting the potential application of this drug-delivery system in the field of biomedicine. (AU)

• Processo FAPESP: 20/03646-2 - Impacto das nanoplataformas baseadas em óxido nítrico e quimioterápicos na citotoxicidade e sensibilização de células tumorais resistentes
• Beneficiário: Joana Claudio Pieretti
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 20/08566-7 - Desenvolvimento de nanopartículas magnéticas de Fe3O4@SiO2 contendo doadores de óxido nítrico para aplicações biomédicas
• Beneficiário: Bianca de Melo Santana
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 22/00321-0 - Nanomateriais aliados a doadores de óxido nítrico para aplicações biológicas
• Beneficiário: Amedea Barozzi Seabra
• Modalidade de apoio: Auxílio à Pesquisa - Regular