First report of sunflower chlorotic mottle virus infecting sunflower plants in Brazil

Autor(es)

Bello, Vinicius Henrique ; Favara, Gabriel Madoglio ; Bernardi, Gustavo Verruma ; Marques Rezende, Jorge Alberto ; Salaroli, Renato Barbosa ; Kitajima, Elliot Watanabe

• Tipo de documento: Artigo Científico
• Fonte: CIENTIA AGRICOL; v. 80, p. 5-pg., 2023-01-01.

Resumo

Sunflower (Helianthus annuus) plants showing symptoms of chlorosis, mosaic, chlorotic ringspot, and necrosis on younger leaves were found in a small experimental plot in Piracicaba, in the state of Sao Paulo, Brazil. Preliminary examinations by transmission electron microscopy of symptomatic leaf tissue revealed flexuous filamentous particles 13-15 nm wide and 700-750 nm long, and cytoplasmatic cylindrical inclusions typical of those found in plant cells infected by members of the Potyvirus genus. Total RNA extracted from symptomatic leaves and subjected to RT-PCR followed by partial nucleotide sequencing confirmed the presence of a potyvirus in the affected plants, which was identified as sunflower chlorotic mottle virus (SuCMoV), a member of the Sunflower chlorotic mottle virus (genus Potyvirus, family Potyviridae) species. Mechanical transmission assays with extracts of symptomatic sunflower leaves reproduced the original symptoms in sunflowers, mosaic symptoms in Zinnia elegans, and chlorotic local lesions in Chenopodium amaranticolor and C. quinoa. Sunflower and zinnia plants became infected after aphid transmission experiments with Myzus persicae. RT-PCR tests using specific primers for SuCMoV confirmed the presence of this virus in experimentally infected plants, meeting the criteria of Koch's postulate. This is the first report of SuCMoV infecting sunflower plants in Brazil. (AU)

• Processo FAPESP: 20/05563-7 - A hipótese do amplifier host na epidemiologia do Mosaico Dourado do tomateiro, causado pelo Tomato Severe Rugose Virus (ToSRV): comprovação experimental e determinação das condições de ocorrência
• Beneficiário: Vinicius Henrique Bello
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/18274-3 - Begomovirus e crinivirus em solanáceas: epidemiologia molecular regional e alternativas sustentáveis de manejo integrado
• Beneficiário: Jorge Alberto Marques Rezende
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 21/03113-7 - Estudos sobre possibilidades de a forese estar envolvida na dispersão de ácaros Brevipalpus e dos vírus que eles transmitem
• Beneficiário: Gustavo Verruma Bernardi
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 18/01633-0 - Tomato severe rugose vírus: potencial de espécies suscetíveis como fonte de inóculo do vírus, transmissão vertical e horizontal por B. tabaci MEAM1 e seleção de estirpes fracas para pré-imunização em tomateiro
• Beneficiário: Gabriel Madoglio Favara
• Modalidade de apoio: Bolsas no Brasil - Doutorado