Insights into the Genomic Potential of a Methylocystis sp. from Amazonian Floodplain Sediments

Autor(es)

Gontijo, Julia B. ; Paula, Fabiana S. ; Venturini, Andressa M. ; Mandro, Jessica A. ; Bodelier, Paul L. E. ; Tsai, Siu M.

• Tipo de documento: Artigo Científico
• Fonte: MICROORGANISMS; v. 10, n. 9, p. 11-pg., 2022-09-01.

Resumo

Although floodplains are recognized as important sources of methane (CH4) in the Amazon basin, little is known about the role of methanotrophs in mitigating CH4 emissions in these ecosystems. Our previous data reported the genus Methylocystis as one of the most abundant methanotrophs in these floodplain sediments. However, information on the functional potential and life strategies of these organisms living under seasonal flooding is still missing. Here, we described the first metagenome-assembled genome (MAG) of a Methylocystis sp. recovered from Amazonian floodplains sediments, and we explored its functional potential and ecological traits through phylogenomic, functional annotation, and pan-genomic approaches. Both phylogenomics and pan-genomics identified the closest placement of the bin.170_fp as Methylocystis parvus. As expected for Type II methanotrophs, the Core cluster from the pan-genome comprised genes for CH4 oxidation and formaldehyde assimilation through the serine pathway. Furthermore, the complete set of genes related to nitrogen fixation is also present in the Core. Interestingly, the MAG singleton cluster revealed the presence of unique genes related to nitrogen metabolism and cell motility. The study sheds light on the genomic characteristics of a dominant, but as yet unexplored methanotroph from the Amazonian floodplains. By exploring the genomic potential related to resource utilization and motility capability, we expanded our knowledge on the niche breadth of these dominant methanotrophs in the Amazonian floodplains. (AU)

• Processo FAPESP: 18/14974-0 - Dinâmica das comunidades microbianas associadas aos ciclos do metano e enxofre em solos amazônicos
• Beneficiário: Júlia Brandão Gontijo
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/50320-4 - Dimensões US-BIOTA - São Paulo: pesquisa colaborativa: integrando as dimensões da biodiversidade microbiana ao longo de áreas de alteração do uso da terra em florestas tropicais
• Beneficiário: Tsai Siu Mui
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 17/26138-0 - Avaliações taxonômica e funcional da comunidade microbiana envolvidas na ciclagem do metano nos solos da Amazônia Brasileira
• Beneficiário: Fabiana da Silva Paula
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/25924-7 - Disentangling the metabolic and ecological interactions between CH4 and S cycles in Amazonian floodplains
• Beneficiário: Júlia Brandão Gontijo
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 19/25931-3 - Avaliação taxonômica e funcional da comunidade microbiana envolvida na ciclagem do metano em solos da Amazônia Brasileira
• Beneficiário: Andressa Monteiro Venturini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado