| Texto completo | |
| Autor(es): |
Nogueira Alves, Ana Paula
;
Arango-Ospina, Marcela
;
Moraes Saldanha Oliveira, Rodrigo Luiz
;
Ferreira, Igor Maia
;
de Moraes, Elisangela Guzi
;
Hartmann, Malte
;
Novaes de Oliveira, Antonio Pedro
;
Boccaccini, Aldo Roberto
;
Triches, Eliandra de Sousa
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS; v. 111, n. 4, p. 14-pg., 2022-11-28. |
| Resumo | |
Bone infection treatment is a significant challenge for the orthopedic field. 3D printing is a promising technology to produce scaffolds with customized architecture, able to stimulate and support bone growth. beta-TCP and S53P4 bioactive glass (BG) are well-known biomaterials for scaffold manufacturing. However, a multifunctional scaffold, able to inhibit microbial proliferation at the defect site, is of increasing interest to avoid infection recurrence. Tea tree oil (TTO) has aroused interest as an antimicrobial agent to minimize the use of antibiotics. Therefore, combining the regenerative potential of a bioceramic with TTO's antimicrobial properties could result in a scaffold capable of stimulating tissue growth and treating infections. In this context, this study aimed to produce and characterize 3D-printed beta-TCP/S53P4 BG scaffolds coated with TTO. Scaffolds morphological and chemical characterizations were carried out through XDR, SEM, and FTIR analysis. beta-TCP/S53P4 BG scaffolds showed a compressive strength of similar to 2 MPa and 53 +/- 2% of porosity. The scaffolds were coated by two different procedures, using an ethanol/TTO (EtOH/TTO) and a gelatin/TTO (Gel/TTO) solution with 5, 10, and 15% (v/v) TTO. The addition of TTO decreased MG-63 cell viability for both coating groups, but the Gel/TTO group showed higher cell viability. The antibacterial activity of the coated scaffolds was evaluated against S. aureus and higher inhibition of colony formation was found for Gel/TTO group. Therefore, the coating with Gel/TTO was effective in terms of antibacterial activity and cell viability. Such Gel/TTO coated beta-TCP/S53P4 BG scaffolds are proposed for antibacterial bone tissue engineering. (AU) | |
| Processo FAPESP: | 19/19187-0 - Produção de scaffolds de biovidro S53P4/alginato de sódio por robocasting: otimização dos parâmetros reológicos da pasta e avaliação da atividade antimicrobiana |
| Beneficiário: | Rodrigo Luiz Moraes Saldanha Oliveira |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 19/08141-9 - Otimização dos parâmetros reológicos de pastas cerâmicas de B-TCP/BG S53P4 visando a produção de scaffolds pelo método de robocasting |
| Beneficiário: | Ana Paula Nogueira Alves |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 19/19594-4 - Impressão 3D de scaffolds bioativos e reabsorvíveis por robocasting: síntese, processamento e atividade antibacteriana |
| Beneficiário: | Eliandra de Sousa Trichês |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 20/12944-7 - Avaliação da atividade antibacteriana e biológica de scaffolds de B-TCP/S53P4 obtidos por robocasting e revestidos com óleo de melaleuca |
| Beneficiário: | Ana Paula Nogueira Alves |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Mestrado |