Pressurized natural deep eutectic solvents: An alternative approach to agro-soy by-products

Autor(es)

Sanchez Bragagnolo, Felipe ; Socas-Rodriguez, Barbara ; Mendiola, Jose A. ; Cifuentes, Alejandro ; Soleo Funari, Cristiano ; Ibanez, Elena

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN NUTRITION; v. 9, p. 14-pg., 2022-09-08.

Resumo

Soybeans are mainly used for food and biodiesel production. It is estimated that soy crops worldwide wilt leave about 651 million metric tons of branches, leaves, pods, and roots on the ground post-harvesting in 2022/23. These by-products might serve as largely available and cheap source of high added-value metabolites, such as ftavonoids, isoflavonoids, and other phenolic compounds. This work aimed to explore green approaches based on the use of pressurized and gas expanded-liquid extraction combined with natural deep eutectic solvents (NADESs) to achieve phenolic-rich extracts from soy by-products. The total phenolic and flavonoid contents of the generated extracts were quantified and compared with conventional solvents and techniques. Pressurized liquid extraction (PLE) with choline chloride/citric acid/water (1:1:11 - molar ratio) at 120 degrees C, 100 bar, and 20 min, resulted in an optimized condition to generate phenolic and flavonoid-rich fractions of soy by-products. The individual parts of soy were extracted under these conditions, with their metabolic profile obtained by UHPLC-ESI-QToF-MS/MS and potential antioxidant properties by ROS scavenging capacity. Extracts of soy roots presented the highest antioxidant capacity (207.48 +/- 40.23 mg AA/g), three times higher than soybean extracts (68.96 +/- 12.30). Furthermore, Hansen solubility parameters (HSPs) were applied to select natural hydrophobic deep eutectic solvents (NaHDES) as substituents for n-heptane to defat soybeans. Extractions applying NaHDES candidates achieved a similar yield and chromatography profile (GC-QToF-MS) to n-heptane extracts. (AU)

• Processo FAPESP: 14/50926-0 - INCT 2014: biodiversidade e produtos naturais
• Beneficiário: Vanderlan da Silva Bolzani
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 20/09500-0 - Extrações verdes dos resíduos da soja ((Glycine max (L.) Merr.)
• Beneficiário: Felipe Sanchez Bragagnolo
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado Direto
• Processo FAPESP: 18/21128-9 - Desenvolvimento de metodologias verdes na investigação química de resíduos de soja (Glycine max (L.) Merr.) e de seus produtos derivados
• Beneficiário: Felipe Sanchez Bragagnolo
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 17/06216-6 - Desenvolvimento de metodologias analíticas verdes na busca por compostos orgânicos de alto valor agregado em resíduos agrícolas
• Beneficiário: Cristiano Soleo de Funari
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 18/01786-1 - EMU concedido no processo 2017/06216-6: cromatógrafo líquido de ultra alta eficiência
• Beneficiário: Cristiano Soleo de Funari
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários