Cytogenetic Mapping of Cattle BAC Probes for the Hypothetical Ancestral Karyotype of the Family Cervidae

Autor(es)

Bernegossi, Agda Maria ; Vozdova, Miluse ; Cernohorska, Halina ; Kubickova, Svatava ; Galindo, David Javier ; Kadlcikova, Dita ; Rubes, Jiri ; Barbanti Duarte, Jose Mauricio

• Tipo de documento: Artigo Científico
• Fonte: Cytogenetic and Genome Research; v. N/A, p. 8-pg., 2022-08-18.

Resumo

Cervids are characterized by their greatest karyotypic diversity among mammals. A great diversity of chromosome numbers in notably similar morphological groups leads to the existence of several complexes of cryptic species and taxonomic uncertainties. Some deer lineages, such as those of Neotropical deer, stand out for a rapid chromosomal reorganization and intraspecific chromosome polymorphisms, which have not been properly explored yet. For that reason, we contribute to the study of deer karyotype diversity and taxonomy by producing and characterizing new molecular cytogenetic markers for the gray brocket deer (Subulo gouazoubira), a deer species that retained the hypothetical ancestral karyotype of Cervidae. We used bacterial artificial chromosome (BAC) clones derived from the cattle genome (Bos taurus) as markers, which were hybridized on S. gouazoubira metaphase chromosomes. In total, we mapped 108 markers, encompassing all gray brocket deer chromosomes, except the Y chromosome. The detailed analysis of fluorescent in situ hybridization results showed 6 fissions and 1 fusion as interchromosomal rearrangements that have separated cattle and gray brocket deer karyotypes. Each group of BAC probes derived from bovine chromosome pairs 1, 2, 5, 6, 8, and 9 showed hybridization signals on 2 different chromosomes, while pairs 28 and 26 are fused in tandem in a single acrocentric chromosome in S. gouazoubira. Furthermore, the BAC markers detected the occurrence of intrachromosomal rearrangements in the S. gouazoubira chromosomes homologous to pair 1 and the X chromosome of cattle. We present a karyotypic map of the 108 new markers, which will be of great importance for future karyotypic evolution studies in cervids and, consequently, help in their conservation and taxonomy resolution. (AU)

• Processo FAPESP: 17/07014-8 - O uso de topotipos atuais para produção de genótipos e citótipos na revisão taxonômica do gênero Mazama: a base para a conservação das espécies
• Beneficiário: José Maurício Barbanti Duarte
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 19/20810-3 - Caracterização de homologias entre sondas BAC bovinas (Bos taurus) e cromossomos de veado-catingueiro (Mazama gouazoubira): novas perspectivas para estudos intra e interespecíficos do gênero Mazama
• Beneficiário: Agda Maria Bernegossi
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado
• Processo FAPESP: 19/06940-1 - O uso da citogenética molecular para estudos evolutivos e taxonômicos em cervídeos
• Beneficiário: José Maurício Barbanti Duarte
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/25769-9 - Caracterização de homologias entre sondas BACs bovinas (Bos taurus) e cromossomos do veado-catingueiro (Mazama gouazoubira): novas perspectivas para estudos intra e interespecíficos do gênero Mazama
• Beneficiário: Agda Maria Bernegossi
• Modalidade de apoio: Bolsas no Brasil - Mestrado