Socs3 ablation in kisspeptin cells partially prevents lipopolysaccharide-induced body weight loss

Autor(es)

Bohlen, Tabata M. ; de Paula, Daniella G. ; Teixeira, Pryscila D. S. ; Mansano, Naira da Silva ; Alves, Guilherme Andrade ; Donato Jr, Jose ; Frazao, Renata

• Tipo de documento: Artigo Científico
• Fonte: CYTOKINE; v. 158, p. 9-pg., 2022-08-17.

Resumo

Many cytokines have been proposed to regulate reproduction due to their actions on hypothalamic kisspeptin cells, the main modulators of gonadotropin-releasing hormone (GnRH) neurons. Hormones such as leptin, pro-lactin and growth hormone are good examples of cytokines that lead to Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway activation, consequently exerting effects in kisspeptin neurons. Different studies have investigated how specific components of the JAK/STAT signaling pathway affect the functions of kisspeptin cells, but the role of the suppressor of cytokine signaling 3 (SOCS3) in mediating cytokine actions in kisspeptin cells remains unknown. Cre-Loxp technology was used in the present study to ablate Socs3 expression in kisspeptin cells (Kiss1/Socs3-KO). Then, male and female control and Kiss1/Socs3-KO mice were evaluated for sexual maturation, energy homeostasis features, and fertility. It was found that hypothalamic Kiss1 mRNA expression is significantly downregulated in Kiss1/Socs3-KO mice. Despite reduced hypothalamic Kiss1 mRNA content, these mice did not present any sexual maturation or fertility impairments. Additionally, body weight gain, leptin sensitivity and glucose homeostasis were similar to control mice. Interestingly, Kiss1/Socs3- KO mice were partially protected against lipopolysaccharide (LPS)-induced body weight loss. Our results suggest that Socs3 ablation in kisspeptin cells partially prevents the sickness behavior induced by LPS, suggesting that kisspeptin cells can modulate energy metabolism in mice in certain situations. (AU)

• Processo FAPESP: 15/14588-5 - Influência da sensibilidade à leptina na maturação sexual de camundongos
• Beneficiário: Tabata Mariz Bohlen
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/21707-1 - Influência da privação alimentar na excitabilidade de neurônios que expressam o gene Kiss1
• Beneficiário: Renata Frazão
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/01318-8 - Sistema nervoso central como um alvo do hormônio do crescimento para a regulação de múltiplas funções biológicas
• Beneficiário: Jose Donato Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/00801-3 - Envolvimento dos neurônios Kiss1 na via neural através da qual o sistema circadiano regula o ciclo ovulatório
• Beneficiário: Regina Silva Paradela
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/03082-6 - Efeitos da administração de glutamato monossódico em neurônios que expressam o gene Kiss1 e sua correlação com distúrbios reprodutivos
• Beneficiário: Daniella Gonçalves de Paula
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/22189-9 - Fatores que alteram a ingestão alimentar modulam a atividade de neurônios Kiss1?
• Beneficiário: Naira da Silva Mansano
• Modalidade de apoio: Bolsas no Brasil - Doutorado