| Texto completo | |
| Autor(es): |
Facchinatto, William M.
;
Araujo, Leandro O.
;
Moraes, Tiago B.
;
Abelha, Thais F.
;
Lima, Thalita H. N.
;
dos Santos, Danilo M.
;
Campana-Filho, Sergio P.
;
Colnago, Luiz A.
;
Caires, Anderson R. L.
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 23, n. 20, p. 16-pg., 2022-10-01. |
| Resumo | |
Multidrug-resistant bacteria represent a global health and economic burden that urgently calls for new technologies to combat bacterial antimicrobial resistance. Here, we developed novel nanocomposites (NCPs) based on chitosan that display different degrees of acetylation (DAs), and conjugated polymer cyano-substituted poly(p-phenylene vinylene) (CNPPV) as an alternative approach to inactivate Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria. Chitosan's structure was confirmed through FT-Raman spectroscopy. Bactericidal and photobactericidal activities of NCPs were tested under dark and blue-light irradiation conditions, respectively. Hydrodynamic size and aqueous stability were determined by DLS, zeta potential (ZP) and time-domain NMR. TEM micrographs of NCPs were obtained, and their capacity of generating reactive oxygen species (ROS) under blue illumination was also characterized. Meaningful variations on ZP and relaxation time T-2 confirmed successful physical attachment of chitosan/CNPPV. All NCPs exhibited a similar and shrunken spherical shape according to TEM. A lower DA is responsible for driving higher bactericidal performance alongside the synergistic effect from CNPPV, lower nanosized distribution profile and higher positive charged surface. ROS production was proportionally found in NCPs with and without CNPPV by decreasing the DA, leading to a remarkable photobactericidal effect under blue-light irradiation. Overall, our findings indicate that chitosan/CNPPV NCPs may constitute a valuable asset for the development of innovative strategies for inactivation and/or photoinactivation of bacteria. (AU) | |
| Processo FAPESP: | 16/20970-2 - Estudo de quitosanas e derivados hidrofílicos por RMN no domínio do tempo |
| Beneficiário: | William Marcondes Facchinatto |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 21/12694-3 - Aplicações das espectroscopias de RMN e infravermelho na análise não-destrutiva de produtos e processos agroindustriais |
| Beneficiário: | Luiz Alberto Colnago |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |