Metronidazole-loaded gold nanoparticles in natural rubber latex as a potential wound dressing

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Borges, Felipe Azevedo ; Drago, Bruno de Camargo ; Baggio, Luis Otavio ; de Barros, Natan Roberto ; Pegorin Brasil, Giovana Sant'Ana ; Scontri, Mateus ; Mussagy, Cassamo Ussemane ; da Silva Ribeiro, Matheus Cicero ; Pereira Milori, Debora Marcondes Bastos ; de Morais, Carla Pereira ; Marangoni, Bruno Spolon ; Nicolodelli, Gustavo ; Mecwan, Marvin ; Mandal, Kalpana ; Guerra, Nayrim Brizuela ; Menegatti, Carlos Renato ; Herculano, Rondinelli Donizetti

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 211, p. 12-pg., 2022-05-18.

Resumo

Gold nanoparticles (AuNPs) have shown interesting properties and specific biofunctions, providing benefits and new opportunities for controlled release systems. In this research, we demonstrated the use of natural rubber latex (NRL) from Hevea brasiliensis as a carrier of AuNPs and the antibiotic metronidazole (MET). We prepared AuNP-MET-NRL and characterized by physicochemical, biological and in vitro release assays. The effect of AuNPs on MET release was evaluated using UV-Vis and Laser-Induced Breakdown Spectroscopy (LIBS) techniques. AuNPs synthesized by Turkevich and Frens method resulted in a spherical shape with diameters of 34.8 +/- 5.5 nm. We verified that there was no emergence or disappearance of new vibrational bands. Qualitatively and quantitatively, we showed that the MET crystals dispersed throughout the NRL. The Young's modulus and elongation values at dressing rupture were in the range appropriate for human skin application. 64.70% of the AuNP-MET complex was released within 100 h, exhibiting a second-order exponential release profile. The LIBS technique allowed monitoring of the AuNP release, indicating the Au emission peak reduction at 267.57 nm over time. Moreover, the dressing displayed an excellent hemocompatibility and fibroblast cell viability. These results demonstrated that the AuNP-MET-NRL wound dressing is a promising approach for dermal applications. (AU)

• Processo FAPESP: 12/12958-1 - Avaliação da liberação controlada de fármaco e nanopartícula utilizando membranas de látex natural como carreador
• Beneficiário: Bruno de Camargo Drago
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/17526-8 - Desenvolvimento de um novo sistema empregando o látex natural como liberador de extratos vegetais reforçados por nanopartículas: caracterização física, química e biológica
• Beneficiário: Rondinelli Donizetti Herculano
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 11/17411-8 - Avaliação da liberação controlada de fármacos utilizando membranas de látex natural como carreador
• Beneficiário: Rondinelli Donizetti Herculano
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 20/08655-0 - Desenvolvimento de uma tecnologia sustentável e biocompatível para a recuperação e purificação da Astaxantina: um poderoso antioxidante de valor econômico
• Beneficiário: Cassamo Ussemane Mussagy
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado