Molecular Epidemiology and Presence of Hybrid Pathogenic Escherichia coli among Isolates from Community-Acquired Urinary Tract Infection

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Nascimento, Jullia A. S. ; Santos, Fernanda F. ; Santos-Neto, Jose F. ; Trovao, Liana O. ; Valiatti, Tiago B. ; Pinaffi, Isabel C. ; Vieira, Monica A. M. ; Silva, Rosa M. ; Falsetti, Ivan N. ; Santos, Ana C. M. ; Gomes, Tania A. T.

• Tipo de documento: Artigo Científico
• Fonte: MICROORGANISMS; v. 10, n. 2, p. 17-pg., 2022-02-01.

Resumo

Urinary tract infections (UTI) affect community and healthcare patients worldwide and may have different clinical outcomes. We assessed the phylogenetic origin, the presence of 43 virulence factors (VFs) of diarrheagenic and extraintestinal pathogenic Escherichia coli, and the occurrence of hybrid strains among E. coli isolates from 172 outpatients with different types of UTI. Isolates from phylogroup B2 (46%) prevailed, followed by phylogroups A (15.7%) and B1 (12.2%), with similar phylogenetic distribution in symptomatic and asymptomatic patients. The most frequent VFs according to their functional category were fimA (94.8%), ompA (83.1%), ompT (63.3%), chuA (57.6%), and vat (22%). Using published molecular criteria, 34.3% and 18.0% of the isolates showed intrinsic virulence and uropathogenic potential, respectively. Two strains carried the eae and escV genes and one the aggR gene, which classified them as hybrid strains. These hybrid strains interacted with renal and bladder cells, reinforcing their uropathogenic potential. The frequency of UPEC strains bearing a more pathogenic potential in the outpatients studied was smaller than reported in other regions. Our data contribute to deepening current knowledge about the mechanisms involved in UTI pathogenesis, especially among hybrid UPEC strains, as these could colonize the host's intestine, leading to intestinal infections followed by UTI. (AU)

• Processo FAPESP: 19/14553-8 - Análise comparativa de características de virulência de Escherichia coli diarreiogênica em cepas de E. coli isoladas de pacientes com diferentes tipos de infecção urinária
• Beneficiário: Júllia Assis da Silva Nascimento
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 19/21685-8 - Estudo de marcadores de virulência presentes em cepas de Escherichia coli isoladas da microbiota intestinal de pacientes com infecção urinária
• Beneficiário: José Francisco Santos Neto
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 17/14821-7 - Explorando novas estratégias de virulência em Escherichia coli
• Beneficiário: Tânia Aparecida Tardelli Gomes do Amaral
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/17353-7 - ANÁLISE COMPARATIVA DE CARACTERÍSTICAS DE VIRULÊNCIA DE Escherichia coli DIARREIOGÊNICA EM CEPAS DE E. coli ISOLADAS DE PACIENTES COM DIFERENTES TIPOS DE INFECÇÃO URINÁRIA
• Beneficiário: Tânia Aparecida Tardelli Gomes do Amaral
• Modalidade de apoio: Auxílio à Pesquisa - Regular