Curcumin-loaded carrageenan nanoparticles: Fabrication, characterization, and assessment of the effects on osteoblasts mineralization

Autor(es)

Nogueira, Lucas Fabricio Bahia ; Cruz, Marcos Antonio Eufrasio ; Tovani, Camila Bussola ; Lopes, Helena Bacha ; Beloti, Marcio Mateus ; Ciancaglini, Pietro ; Bottini, Massimo ; Ramos, Ana Paula

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 217, p. 9-pg., 2022-09-01.

Resumo

The use of Curcumin (CR) as a bioactive molecule to prevent and treat inflammation- related diseases is widespread. However, the high hydrophobicity hinders the in vivo bioavailability of CR, reducing its therapeutic index. In the present study, we described the use of nanoparticles (NPs) made of kappa-carrageenan (kappa-Carr), a sulphated polysaccharide, as cost-effective, biodegradable and biocompatible CR carriers. CR-loaded kappa-Carr nanoparticles (CR@Carr NPs) were prepared by mixing a kappa-Carr aqueous solution with a CR ethanolic solution. The final suspension was centrifuged and re-suspended in phosphate buffer solution. The NPs' size was tuned by changing the concentration of the polysaccharide. CR@CarrNPs displayed high CR incorporation efficiency (similar to 80 wt%) and a double-exponential curve of CR release at physiological conditions (37 degrees C and pH 7.4) with a cumulative drug release of 32 wt% after 24 h for the smaller NP. Our results also showed that CR@CarrNPs were not cytotoxic to osteoblasts at concentrations up to 1 mu M. Confocal microscopy images revealed the internalization of CR by the cells guided by the NPs. Cells treated with CR@CarrNPs exhibited higher activity of alkaline phosphatase and higher expression of the main osteogenic genes (Sp7, Col1 and Runx2), and mineralized the extracellular matrix in a higher extent compared to the cells cultivated in absence of the NPs. We posited that these effects were related to the NP-driven internalization of CR by osteoblasts. Our study sheds light on the possible use of CR@CarrNPs as efficient and safe therapeutic tools for the treatment of bone-related diseases. (AU)

• Processo FAPESP: 19/25054-2 - Nanopartículas contendo estrôncio e sua versatilidade na fabricação de biomateriais: implicações e aplicações em biomineralização
• Beneficiário: Ana Paula Ramos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/08568-2 - Investigação do papel de vesículas extracelulares (VEs) na iniciação, propagação, regeneração e modelação da mineralização biológica
• Beneficiário: Pietro Ciancaglini
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/25871-8 - Scaffolds constituídos por vidros bioativos e matrizes injetáveis para regeneração óssea
• Beneficiário: Lucas Fabrício Bahia Nogueira
• Modalidade de apoio: Bolsas no Brasil - Doutorado