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Co-Functionalization of Gold Nanoparticles with C7H2 and HuAL1 Peptides: Enhanced Antimicrobial and Antitumoral Activities

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Autor(es):
Formaggio, Daniela M. D. ; Magalhaes, Jessica A. ; Andrade, Vitor M. ; Conceicao, Katia ; Anastacio, Juliana M. ; Santiago, Gabrielli S. ; Arruda, Denise C. ; Tada, Dayane B.
Número total de Autores: 8
Tipo de documento: Artigo Científico
Fonte: PHARMACEUTICS; v. 14, n. 7, p. 17-pg., 2022-07-01.
Resumo

The functionalization of nanoparticles with therapeutic peptides has been pointed out as a promising strategy to improve the applications of these molecules in the field of health sciences. Peptides are highly bioactive but face several limitations such as low bioavailability due to the difficulty of overcoming the physiological barriers in the body and their degradation by enzymes. In this work, gold nanoparticles (AuNPs) were co-functionalized with two therapeutic peptides simultaneously. The peptides from the complementary determining region of monoclonal antibodies, composed of the amino acid sequences YISCYNGATSYNQKFK (C7H2) and RASQSVSSYLA (HuAL1) were chosen for having exhibited antitumor and antimicrobial activity before. The peptides-conjugated AuNPs were characterized regarding size, morphology, and metal concentration by using TEM, dynamic light scattering, and ICP-OES techniques. Then, peptides-conjugated AuNPs were evaluated regarding the antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. The antitumoral activity was evaluated in vitro by cell viability assays with metastatic melanoma cell line (B16F10-Nex2) and the cytotoxicity was evaluated against human foreskin fibroblast (Hs68) cell line. Finally, in vivo assays were performed by using a syngeneic animal model of metastatic melanoma. Our findings have highlighted the potential application of the dual-peptide AuNPs in order to enhance the antitumor and antimicrobial activity of peptides. (AU)

Processo FAPESP: 18/22890-1 - Síntese e funcionalização de nanopartículas metálicas e bimetálicas para modulação da fotoatividade de fotossensibilizadores
Beneficiário:Jéssica Aparecida Magalhães
Modalidade de apoio: Bolsas no Brasil - Doutorado
Processo FAPESP: 17/01697-6 - Síntese e funcionalização de nanopartículas bimetálicas para aplicação na terapia fotodinâmica de câncer
Beneficiário:Dayane Batista Tada
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 18/11972-7 - Análise da Expressão de Proteínas Reguladas pelo Fator de Transcrição Brn-2 em Células Tratadas com Peptídeos Derivados do Domínio POU da Proteína Brn-2 e Determinação da Internalização e Localização Celular desses Peptídeos
Beneficiário:Denise Costa Arruda
Modalidade de apoio: Auxílio à Pesquisa - Regular