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A look into Phaffia rhodozyma biorefinery: From the recovery and fractionation of carotenoids, lipids and proteins to the sustainable manufacturing of biologically active bioplastics

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Mussagy, Cassamo U. ; Remonatto, Daniela ; Picheli, Flavio P. ; V. Paula, Ariela ; Herculano, Rondinelli D. ; Santos-Ebinuma, Valeria C. ; Farias, Renan L. ; Onishi, Bruno S. D. ; Ribeiro, Sidney J. L. ; Pereira, Jorge F. B. ; Pessoa, Adalberto
Número total de Autores: 11
Tipo de documento: Artigo Científico
Fonte: Bioresource Technology; v. 362, p. 11-pg., 2022-08-19.
Resumo

Carotenoids over-producing yeast has become a focus of interest of the biorefineries, in which the integration of the bioproduction with the following downstream processing units for the recovery and purification of carot-enoids and other value-added byproducts is crucial to improve the sustainability and profitability of the overall bioprocess. Aiming the future implementation of Phaffia rhodozyma-based biorefineries, in this work, an inte-grative process for fractionation of intracellular compounds from P. rhodozyma biomass using non-hazardous bio-based solvents was developed. After one-extraction step, the total amount of astaxanthin, beta-carotene, lipids and proteins recovered was 63.11 mu g/gDCW, 42.81 mu g/gDCW, 53.75 mg/gDCW and 10.93 mg/g, respectively. The implementation of sequential back-extraction processes and integration with saponification and precipitation operations allowed the efficient fractionation and recovery (% w/w) of astaxanthin (-72.5 %), beta-carotene-90.17 %), proteins (21.04 %) and lipids (23.72 %). After fractionation, the manufacture of carotenoids-based products was demonstrated, through the mixture of carotenoids-rich extracts with bacterial cellulose to obtain biologically active bioplastics. (AU)

Processo FAPESP: 19/15493-9 - Desenvolvimento de hidrogéis eletrocondutivos baseados em líquidos iônicos
Beneficiário:Valéria de Carvalho Santos Ebinuma
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 20/08655-0 - Desenvolvimento de uma tecnologia sustentável e biocompatível para a recuperação e purificação da Astaxantina: um poderoso antioxidante de valor econômico
Beneficiário:Cassamo Ussemane Mussagy
Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 21/06686-8 - Processo biotecnológico visando o desenvolvimento de colorantes naturais microbianos para aplicação industrial: fase II
Beneficiário:Valéria de Carvalho Santos Ebinuma
Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Jovens Pesquisadores - Fase 2