Standardization proposal to quality control of propolis extracts commercialized in Brazil : A fingerprinting methodology using a UHPLC-PDA-MS/MS approach

Autor(es)

Contieri, Leticia S. ; de Souza Mesquita, Leonardo M. ; Sanches, Vitor L. ; Vigano, Juliane ; Martinez, Julian ; da Cunha, Diogo T. ; Rostagno, Mauricio A.

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 161, p. 11-pg., 2022-08-30.

Resumo

Propolis is a rich source of known and largely explored bioactive compounds with many pharmacological properties. It is used in several commercialized products, such as propolis-enriched honey, candies, mouth and throat sprays, soaps, toothpaste, and skin creams. However, the great diversity of propolis products and different types make the standardization of realistic quality control procedures challenging. Moreover, the extraction of propolis bioactive compounds depends on the technique and the solvent used. In Brazil, the Ministry of Agri-culture, Livestock, and Supply (MAPA) set standards to establish commercialized propolis extracts' identity and quality. In addition, according to legislation, propolis extracts must present the main classes of phenols at 200 and 400 nm on the UV spectrum. Still, it is not specified which analysis method should be used to guarantee feasible quality control of the commercialized samples. For this, we proposed a new fast UHPLC-PDA-MS/MS method for analysis and quantification of propolis phenolic compounds. Moreover, we hypothesize that there is no efficient monitoring regarding the quality of the propolis extracts sold in Brazilian stores. Therefore, the present study aimed to perform quality control of 17 Brazilian propolis extracts produced in the Southeast region (green or brown - the most representative samples). The dry extract content (% g/mL), oxidation index (sec-onds), total flavonoids, and phenolics (% m/m) of each sample were compared with legislation. We conclude that using the UHPLC-PDA method and the investigation that allowed the comparison with the current legislation efficiently practical problems in the commercialization of propolis extracts. However, of the 17 analyzed sam-ples, 6 did not meet the desired the recognized standards, denoting a lack of supervision and efficient quality control, which highlights a dangerous situation regarding the commercialization of this critical product used in several industrial fields, mainly in the food and pharmaceutical sector. (AU)

• Processo FAPESP: 18/14582-5 - Desenvolvimento de um sistema bidimensional de extração, separação e análise para avaliação da bioatividade de fitoquímicos
• Beneficiário: Maurício Ariel Rostagno
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores - Fase 2
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• Beneficiário: Leonardo Mendes de Souza Mesquita
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
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• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado