Aging reduces ABHD5 protein content in the adipose tissue of mice: The reversal effect of exercise

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Bricola, Rafael S. ; Cordeiro, Andre, V ; Crisol, Barbara M. ; Braga, Renata R. ; de Melo, Diego G. ; Rocha, Matheus B. ; Gaspar, Rafael C. ; Nakandakari, Susana C. B. R. ; Silva, Vagner R. R. ; Anaruma, Chadi P. ; Katashima, Carlos K. ; Canciglieri, Raphael D. S. ; Munoz, Vitor R. ; Pavan, Isadora C. B. ; Pinto, Ana P. ; Simabuco, Fernando M. ; da Silva, Adelino S. R. ; Moura, Leandro P. ; Pauli, Jose R. ; Cintra, Dennys E. ; Ropelle, Eduardo R.

• Tipo de documento: Artigo Científico
• Fonte: Cell Biochemistry and Function; v. N/A, p. 10-pg., 2022-12-14.

Resumo

Dysfunction of the adipose tissue metabolism is considered as a significant hallmark of aging. It has been proposed that alpha-beta hydrolase domain containing 5 (ABHD5) plays a critical role in the control of lipolysis. However, the role of ABHD5 in the control of lipolysis during aging or exercise is unknown. Here we combined the experimental mouse model with transcriptomic analyzes by using murine and human databases to explore the role of ABHD5 in the adipose tissue during aging and in response to exercise. Transcriptomic data revealed a downregulation of Abhd5 messenger RNA levels in the subcutaneous white adipose tissue (scWAT) over time in individuals from 20 to 69 years old. Aged mice displayed dramatic reduction of ABHD5 protein content and lipolytic-related proteins in the scWAT. Interestingly, 4 weeks of high-intensity interval training increased ABHD5 protein level and restored the lipolytic pathway in the scWAT of aged mice. Altogether, our findings demonstrated that aging affects ABHD5 content in the adipose tissue of mice and humans. Conversely, exercise increases ABHD5 activity, recovering the lipolytic activity in aged mice. (AU)

• Processo FAPESP: 19/21709-4 - Implicações do desequilíbrio mitonuclear e da UPRmt em neurônios hipotalâmicos na gênese da Obesidade
• Beneficiário: Eduardo Rochete Ropelle
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/03122-3 - Expressão de ABHD5 em tecido adiposo no envelhecimento e a influência do exercício físico em sua atividade
• Beneficiário: Rafael dos Santos Brícola
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 19/11820-5 - Função do Nr1d1 sobre a Sarcopenia associada ao envelhecimento
• Beneficiário: Adelino Sanchez Ramos da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Temático